› 2fib

X-ray diffraction
2.01Å resolution

RECOMBINANT HUMAN GAMMA-FIBRINOGEN CARBOXYL TERMINAL FRAGMENT (RESIDUES 143-411) COMPLEXED TO THE PEPTIDE GLY-PRO-ARG-PRO AT PH 6.0

Released:
DOI: 10.2210/pdb2fib/pdb
PDB entry 2fib coloured by chain, front view.
PDB entry 2fib coloured by chain, side view.
PDB entry 2fib coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The primary fibrin polymerization pocket: three-dimensional structure of a 30-kDa C-terminal gamma chain fragment complexed with the peptide Gly-Pro-Arg-Pro.
Pratt KP, Côté HC, Chung DW, Stenkamp RE, Davie EW
Proc Natl Acad Sci U S A 94 7176-81 (1997)
PMID: 9207064

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136042 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fibrinogen gamma chain Chain: A
Molecule details ›
Chain: A
Length: 269 amino acids
Theoretical weight: 30.24 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P02679 (Residues: 169-433; Coverage: 62%)
Gene names: FGG, PRO2061
Sequence domains: Fibrinogen beta and gamma chains, C-terminal globular domain
Structure domains:
GLY-PRO-ARG-PRO Chain: B
Molecule details ›
Chain: B
Length: 4 amino acids
Theoretical weight: 426 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P21
Unit cell:
a: 37.33Å b: 68.2Å c: 47.6Å
α: 90° β: 105.02° γ: 90°
R-values:
R R work R free
0.186 0.186 0.282
Expression systems:
  • Komagataella pastoris
  • Not provided

Quick links

Citations

14 review citations

Fibrinogen and fibrin structure and functions.
Mosesson MW. (2005)
13 more

7 mentions without citation

The primary fibrin polymerization pocket: three-dimensional structure of a 30-kDa C-terminal gamma chain fragment complexed with the peptide Gly-Pro-Arg-Pro.
Pratt et al. (1997)
6 more