› 2fcq

X-ray diffraction
3.3Å resolution

X-ray Crystal Structure of a Chemically Synthesized Ubiquitin with a Cubic Space Group

Released:
DOI: 10.2210/pdb2fcq/pdb
PDB entry 2fcq coloured by chain, front view.
PDB entry 2fcq coloured by chain, side view.
PDB entry 2fcq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Dissecting the energetics of protein alpha-helix C-cap termination through chemical protein synthesis.
Bang D, Gribenko AV, Tereshko V, Kossiakoff AA, Kent SB, Makhatadze GI
Nat Chem Biol 2 139-43 (2006)
PMID: 16446709

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192942 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin-like domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 76 amino acids
Theoretical weight: 8.56 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9PST8 (Residues: 1-71; Coverage: 100%)
Gene name: UbI
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

1 bound ligand:
Ligand CD 7 x CD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P4332
Unit cell:
a: 105.059Å b: 105.059Å c: 105.059Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.241 0.286
Expression system: Not provided

Quick links

Citations

8 review citations

Total chemical synthesis of proteins.
Kent SB. (2009)
7 more

4 mentions without citation

Discovering conformational sub-states relevant to protein function.
Ramanathan et al. (2011)
3 more