PDBe 2f3w

Solution NMR

solution structure of 1-110 fragment of staphylococcal nuclease in 2M TMAO

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TNase-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 110 amino acids
Theoretical weight: 12.33 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A5A513 (Residues: 69-178; Coverage: 66%)
Gene name: nuc
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 41%
Refinement method: simulated annealing torsion angle dynamics
Chemical shifts: BMR6908  
Expression system: Escherichia coli BL21(DE3)