PDBe 2f0w

X-ray diffraction
2.1Å resolution

Crystal structure of Staphylococcal nuclease mutant V23I/L25I/V66L/I72L

Released:
Source organism: Staphylococcus aureus
Entry authors: Lu JZ, Sakon J, Stites WE

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nuclease A Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.87 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Best match: P00644 (Residues: 83-231)
Gene name: nuc

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P41
Unit cell:
a: 48.275Å b: 48.275Å c: 63.789Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.277
Expression system: Escherichia coli