PDBe 2f0h

X-ray diffraction
2.15Å resolution

Crystal structure of Staphylococcal nuclease mutant V66L

Released:
Source organism: Staphylococcus aureus
Entry authors: Lu JZ, Sakon J, Stites WE

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nuclease A Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 16.86 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Best match: P00644 (Residues: 83-231)
Gene name: nuc

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P41
Unit cell:
a: 47.84Å b: 47.84Å c: 63.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.197 0.282
Expression system: Escherichia coli