Solution NMR

The NMR ensemble structure of the Itk SH2 domain bound to a phosphopeptide


Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Structure domain:

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein kinase ITK/TSK Chain: A
Molecule details ›
Chain: A
Length: 109 amino acids
Theoretical weight: 12.56 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q03526 (Residues: 238-344; Coverage: 17%)
Gene names: Emt, Itk, Tlk, Tsk
Sequence domains: SH2 domain
Structure domains: SH2 domain
Lymphocyte cytosolic protein 2 Chain: B
Molecule details ›
Chain: B
Length: 8 amino acids
Theoretical weight: 795 Da
Source organism: Mus musculus
Expression system: Not provided
  • Canonical: Q60787 (Residues: 143-148; Coverage: 1%)
Gene name: Lcp2

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
Refinement method: Distance geometry simulated annealing was used for refinement
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided