2eo0

X-ray diffraction
2.4Å resolution

Crystal Structure of Holliday Junction Resolvase ST1444

Released:
Entry authors: Sarai N, Kagawa W, Kurumizaka H, Yokoyama S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction)
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Holliday junction resolvase Hjc Chains: A, B
Molecule details ›
Chains: A, B
Length: 147 amino acids
Theoretical weight: 16.74 KDa
Source organism: Sulfurisphaera tokodaii str. 7
Expression system: Escherichia coli
UniProt:
  • Canonical: F9VND5 (Residues: 1-144; Coverage: 100%)
Gene names: STK_14440, hjc
Sequence domains: Archaeal holliday junction resolvase (hjc)
Structure domains: Trna Endonuclease; Chain: A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 44.661Å b: 49.326Å c: 69.894Å
α: 90° β: 103.28° γ: 90°
R-values:
R R work R free
0.312 0.232 0.312
Expression system: Escherichia coli