2e7l

X-ray diffraction
2.5Å resolution

Structure of a high-affinity mutant of the 2C TCR in complex with Ld/QL9

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Ig-like domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 113 amino acids
Theoretical weight: 12.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A2NTU7 (Residues: 21-132; Coverage: 45%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chains: C, D
Molecule details ›
Chains: C, D
Length: 121 amino acids
Theoretical weight: 13.27 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A2NTY6 (Residues: 30-144; Coverage: 96%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
H-2 class I histocompatibility antigen, L-D alpha chain Chains: E, F
Molecule details ›
Chains: E, F
Length: 181 amino acids
Theoretical weight: 21.21 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01897 (Residues: 25-205; Coverage: 54%)
Gene name: H2-L
Sequence domains: Class I Histocompatibility antigen, domains alpha 1 and 2
Structure domains: MHC class I-like antigen recognition-like
Peptide (GLN)(LEU)(SER)(PRO)(PHE)(PRO)(PHE)(ASP)(LEU) Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 9 amino acids
Theoretical weight: 1.06 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P42212
Unit cell:
a: 113.54Å b: 113.54Å c: 177.452Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.224 0.246
Expression system: Escherichia coli BL21