X-ray diffraction
2.2Å resolution

5-methyltetrahydrofolate corrinoid/iron sulfur protein methyltransferase complexed with methyltetrahydrofolate to 2.2 Angsrom resolution


Function and Biology Details

Reaction catalysed:
A [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrofolate = a [Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrofolate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
5-methyltetrahydrofolate:corrinoid/iron-sulfur protein co-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 262 amino acids
Theoretical weight: 28.64 KDa
Source organism: Moorella thermoacetica
Expression system: Escherichia coli
  • Canonical: Q46389 (Residues: 1-262; Coverage: 100%)
Gene name: acsE
Sequence domains: Pterin binding enzyme
Structure domains: Dihydropteroate synthase-like

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 50.159Å b: 78.547Å c: 135.617Å
α: 90° β: 90° γ: 90°
R R work R free
0.178 0.172 0.23
Expression system: Escherichia coli