2e2a

X-ray diffraction
2.1Å resolution

ASP81LEU ENZYME IIA FROM THE LACTOSE SPECIFIC PTS FROM LACTOCOCCUS LACTIS

Released:
Source organism: Lactococcus lactis
Entry authors: Sliz P, Koch B, Hengstenberg W, Pai EF

Function and Biology Details

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PTS system lactose-specific EIIA component Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 105 amino acids
Theoretical weight: 11.46 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: P23532 (Residues: 1-105; Coverage: 100%)
Gene name: lacF
Sequence domains: PTS system, Lactose/Cellobiose specific IIA subunit
Structure domains: Phosphotransferase system, lactose/cellobiose-type IIA subunit

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P41212
Unit cell:
a: 87.96Å b: 87.96Å c: 79.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.198 0.249
Expression system: Escherichia coli