2dt7

Solution NMR

Solution structure of the second SURP domain of human splicing factor SF3a120 in complex with a fragment of human splicing factor SF3a60

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Splicing factor 3A subunit 3 Chain: A
Molecule details ›
Chain: A
Length: 38 amino acids
Theoretical weight: 4.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12874 (Residues: 71-107; Coverage: 7%)
Gene names: SAP61, SF3A3
Sequence domains: Splicing factor SF3a60 binding domain
Splicing factor 3A subunit 1 Chain: B
Molecule details ›
Chain: B
Length: 85 amino acids
Theoretical weight: 9.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15459 (Residues: 134-217; Coverage: 11%)
Gene names: SAP114, SF3A1
Sequence domains: Surp module
Structure domains: Surp module

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: TORSION ANGLE DYNAMICS, RESTRAINTED ENERGY MINIMIZATION
Chemical shifts: BMR11365  
Expression system: Escherichia coli