PDBe 2ddr

X-ray diffraction
1.4Å resolution

Crystal structure of sphingomyelinase from Bacillus cereus with calcium ion

Released:

Function and Biology Details

Reaction catalysed:
Sphingomyelin + H(2)O = N-acylsphingosine + phosphocholine
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sphingomyelinase C Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 306 amino acids
Theoretical weight: 34.34 KDa
Source organism: Bacillus cereus
Expression system: Bacillus subtilis
UniProt:
  • Canonical: P11889 (Residues: 28-333; Coverage: 100%)
Gene name: sph
Sequence domains: Endonuclease/Exonuclease/phosphatase family
Structure domains: Endonuclease/exonuclease/phosphatase

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45XU
Spacegroup: P1
Unit cell:
a: 65.43Å b: 72.691Å c: 77.859Å
α: 112.19° β: 89.97° γ: 116.76°
R-values:
R R work R free
0.213 0.213 0.234
Expression system: Bacillus subtilis