› 2dcy

X-ray diffraction
1.4Å resolution

Crystal structure of Bacillus subtilis family-11 xylanase

Released:
DOI: 10.2210/pdb2dcy/pdb
PDB entry 2dcy coloured by chain, front view.
PDB entry 2dcy coloured by chain, side view.
PDB entry 2dcy coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Thermal stabilization of Bacillus subtilis family-11 xylanase by directed evolution.
Miyazaki K, Takenouchi M, Kondo H, Noro N, Suzuki M, Tsuda S
J Biol Chem 281 10236-42 (2006)
PMID: 16467302

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148365 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endo-1,4-beta-xylanase A Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 185 amino acids
Theoretical weight: 20.39 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P18429 (Residues: 29-213; Coverage: 100%)
Gene names: BSU18840, xynA
Sequence domains: Glycosyl hydrolases family 11
Structure domains: Jelly Rolls

Ligands and Environments

3 bound ligands:
Ligand DIO 3 x DIO
Ligand TLA 6 x TLA
Ligand TAR 1 x TAR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P21212
Unit cell:
a: 130.445Å b: 186.209Å c: 37.979Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.197 0.217
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Directed evolution: an approach to engineer enzymes.
Kaur et al. (2006)
3 more

3 mentions without citation

Protein-protein docking benchmark version 3.0.
Hwang et al. (2008)
2 more