› 2cyb

X-ray diffraction
1.8Å resolution

Crystal structure of Tyrosyl-tRNA Synthetase complexed with L-tyrosine from Archaeoglobus fulgidus

Released:
DOI: 10.2210/pdb2cyb/pdb
PDB entry 2cyb coloured by chain, front view.
PDB entry 2cyb coloured by chain, side view.
PDB entry 2cyb coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
Crystal structures of tyrosyl-tRNA synthetases from Archaea.
Kuratani M, Sakai H, Takahashi M, Yanagisawa T, Kobayashi T, Murayama K, Chen L, Liu ZJ, Wang BC, Kuroishi C, Kuramitsu S, Terada T, Bessho Y, Shirouzu M, Sekine S, Yokoyama S
J Mol Biol 355 395-408 (2006)
PMID: 16325203

Function and Biology Details

Reaction catalysed: 
ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128130 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine--tRNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 323 amino acids
Theoretical weight: 36.67 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O29482 (Residues: 1-323; Coverage: 100%)
Gene names: AF_0776, tyrS
Sequence domains: tRNA synthetases class I (W and Y)
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand TYR 2 x TYR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 40.62Å b: 96.158Å c: 92.636Å
α: 90° β: 94.41° γ: 90°
R-values:
R R work R free
0.203 0.202 0.24
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Adenylate-forming enzymes.
Schmelz et al. (2009)
4 more

4 mentions without citation

Cyclodipeptide synthases, a family of class-I aminoacyl-tRNA synthetase-like enzymes involved in non-ribosomal peptide synthesis.
Sauguet et al. (2011)
3 more