2c7m

X-ray diffraction
2.4Å resolution

Human Rabex-5 residues 1-74 in complex with Ubiquitin

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rab5 GDP/GTP exchange factor Chain: A
Molecule details ›
Chain: A
Length: 74 amino acids
Theoretical weight: 8.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UJ41 (Residues: 1-74; Coverage: 15%)
Gene names: RABEX5, RABGEF1
Ubiquitin Chain: B
Molecule details ›
Chain: B
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P0CH28 (Residues: 609-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P61
Unit cell:
a: 81.9Å b: 81.9Å c: 55.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.183 0.214
Expression system: Escherichia coli