PDBe 2c5r

X-ray diffraction
2.9Å resolution

The structure of phage phi29 replication organizer protein p16.7 in complex with double stranded DNA

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA replication protein 16.7 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 67 amino acids
Theoretical weight: 7.94 KDa
Source organism: Bacillus virus phi29
Expression system: Escherichia coli
UniProt:
  • Canonical: P16517 (Residues: 64-130; Coverage: 52%)
Gene name: 16.7
Structure domains: Helicase, Ruva Protein; domain 3
5'-D(*TP*CP*CP*AP*CP*CP*GP*GP)-3' Chain: Y
Molecule details ›
Chain: Y
Length: 8 nucleotides
Theoretical weight: 2.39 KDa
Source organism: Bacillus virus phi29
Expression system: Not provided
5'-D(*CP*CP*GP*GP*TP*GP*GP*AP)-3' Chain: Z
Molecule details ›
Chain: Z
Length: 8 nucleotides
Theoretical weight: 2.47 KDa
Source organism: Bacillus virus phi29
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: P212121
Unit cell:
a: 65.505Å b: 72.111Å c: 127.36Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.254 0.251 0.292
Expression systems:
  • Escherichia coli
  • Not provided