PDBe 2c5i

X-ray diffraction
2.3Å resolution

N-terminal domain of tlg1 complexed with N-terminus of vps51 in distorted conformation

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Vacuolar protein sorting-associated protein 51 Chain: P
Molecule details ›
Chain: P
Length: 29 amino acids
Theoretical weight: 3.52 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P36116 (Residues: 2-30; Coverage: 18%)
Gene names: API3, VPS51, VPS67, WHI6, YKR020W
t-SNARE affecting a late Golgi compartment protein 1 Chain: T
Molecule details ›
Chain: T
Length: 101 amino acids
Theoretical weight: 12.01 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q03322 (Residues: 1-101; Coverage: 45%)
Gene names: D8035.11, TLG1, YDR468C
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3221
Unit cell:
a: 65.776Å b: 65.776Å c: 62.577Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.229 0.226 0.296
Expression systems:
  • Not provided
  • Escherichia coli BL21