2c2v

X-ray diffraction
2.9Å resolution

Crystal structure of the CHIP-UBC13-UEV1a complex

Released:

Function and Biology Details

Reactions catalysed:
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Cellular component:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ubiquitin-conjugating enzyme E2 N Chains: B, E, H, K
Molecule details ›
Chains: B, E, H, K
Length: 154 amino acids
Theoretical weight: 17.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61088 (Residues: 2-152; Coverage: 99%)
Gene names: BLU, UBE2N
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme
Ubiquitin-conjugating enzyme E2 variant 1 Chains: C, F, I, L
Molecule details ›
Chains: C, F, I, L
Length: 142 amino acids
Theoretical weight: 16.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13404 (Residues: 7-147; Coverage: 96%)
  • Best match: Q13404-1 (Residues: 80-221)
Gene names: CROC1, P/OKcl.19, UBE2V, UBE2V1, UEV1
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme
STIP1 homology and U box-containing protein 1 Chains: S, T, U, V
Molecule details ›
Chains: S, T, U, V
Length: 78 amino acids
Theoretical weight: 9.03 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WUD1 (Residues: 227-304; Coverage: 26%)
Gene names: Chip, Stub1
Sequence domains: U-box domain
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: C2
Unit cell:
a: 180.31Å b: 69.991Å c: 204.477Å
α: 90° β: 106.95° γ: 90°
R-values:
R R work R free
0.218 0.214 0.297
Expression system: Escherichia coli