PDBe 2c23

X-ray diffraction
2.65Å resolution

14-3-3 Protein Beta (Human) in complex with exoenzyme S peptide

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein beta/alpha Chain: A
Molecule details ›
Chain: A
Length: 245 amino acids
Theoretical weight: 28.24 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31946 (Residues: 1-240; Coverage: 98%)
Gene name: YWHAB
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
Exoenzyme S Chain: P
Molecule details ›
Chain: P
Length: 11 amino acids
Theoretical weight: 1.12 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Not provided
UniProt:
  • Canonical: Q51451 (Residues: 421-431; Coverage: 2%)
Gene name: exoS

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: C2221
Unit cell:
a: 59.908Å b: 86.841Å c: 121.814Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.221 0.286
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided