PDBe 2bri

X-ray diffraction
3Å resolution

UMP KINASE FROM PYROCOCCUS FURIOSUS COMPLEXED WITH ITS SUBSTRATE ANALOG AMPPNP

Released:

Function and Biology Details

Reaction catalysed:
ATP + UMP = ADP + UDP
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridylate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 225 amino acids
Theoretical weight: 24.71 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8U122 (Residues: 1-225; Coverage: 100%)
Gene names: PF1407, pyrH
Sequence domains: Amino acid kinase family
Structure domains: Acetylglutamate kinase-like

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: I23
Unit cell:
a: 146.684Å b: 146.684Å c: 146.684Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.217 0.282
Expression system: Escherichia coli BL21(DE3)