PDBe 2bmu

X-ray diffraction
2.55Å resolution

UMP KINASE FROM PYROCOCCUS FURIOSUS COMPLEXED WITH ITS SUBSTRATE UMP AND ITS SUBSTRATE ANALOG AMPPNP

Released:

Function and Biology Details

Reaction catalysed:
ATP + UMP = ADP + UDP
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridylate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 226 amino acids
Theoretical weight: 24.78 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8U122 (Residues: 1-225; Coverage: 100%)
Gene names: PF1407, pyrH
Sequence domains: Amino acid kinase family
Structure domains: Acetylglutamate kinase-like

Ligands and Environments

3 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: I23
Unit cell:
a: 144.564Å b: 144.564Å c: 144.564Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.191 0.241
Expression system: Escherichia coli BL21(DE3)