2bdp

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF BACILLUS DNA POLYMERASE I FRAGMENT COMPLEXED TO 9 BASE PAIRS OF DUPLEX DNA

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase I Chain: A
Molecule details ›
Chain: A
Length: 580 amino acids
Theoretical weight: 66.2 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P52026 (Residues: 297-876; Coverage: 66%)
Gene names: pol, polA
Sequence domains: DNA polymerase family A
Structure domains:
DNA (5'-D(*GP*CP*AP*TP*GP*AP*TP*GP*C)-3') Chain: P
Molecule details ›
Chain: P
Length: 9 nucleotides
Theoretical weight: 2.76 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided
DNA (5'-D(P*AP*GP*CP*AP*TP*CP*AP*TP*GP*C)-3') Chain: T
Molecule details ›
Chain: T
Length: 10 nucleotides
Theoretical weight: 3.03 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 86.241Å b: 93.275Å c: 106.374Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.241 0.276
Expression systems:
  • Escherichia coli
  • Not provided