PDBe 2bc9

X-ray diffraction
2.8Å resolution

Crystal-structure of the N-terminal large GTPase Domain of human Guanylate Binding protein 1 (hGBP1) in complex with non-hydrolysable GTP analogue GppNHp

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanylate-binding protein 1 Chain: A
Molecule details ›
Chain: A
Length: 328 amino acids
Theoretical weight: 37.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P32455 (Residues: 1-317; Coverage: 54%)
Gene name: GBP1
Sequence domains: Guanylate-binding protein, N-terminal domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: I212121
Unit cell:
a: 54.884Å b: 101.104Å c: 149.261Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.234 0.269
Expression system: Escherichia coli BL21