› 2b7x

X-ray diffraction
3Å resolution

Sequential reorganization of beta-sheet topology by insertion of a single strand

Released:
DOI: 10.2210/pdb2b7x/pdb
PDB entry 2b7x coloured by chain, front view.
PDB entry 2b7x coloured by chain, side view.
PDB entry 2b7x coloured by chain, top view.
Source organism: Tequatrovirus T4
Primary publication:
Sequential reorganization of beta-sheet topology by insertion of a single strand.
Sagermann M, Baase WA, Matthews BW
Protein Sci 15 1085-92 (2006)
PMID: 16597830

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133021 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 170 amino acids
Theoretical weight: 19.23 KDa
Source organism: Tequatrovirus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P00720 (Residues: 1-30, 31-164, 1-24, 25-164; Coverage: 100%)
Gene name: E
Sequence domains: Phage lysozyme
Structure domains: Lysozyme

Ligands and Environments

1 bound ligand:
Ligand SO4 8 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 61.38Å b: 78.08Å c: 143.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.239 0.319
Expression system: Escherichia coli

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Citations

6 citation in other articles

Systematic analysis of short internal indels and their impact on protein folding.
Kim et al. (2010)
5 more