2asb

X-ray diffraction
1.5Å resolution

Structure of a Mycobacterium tuberculosis NusA-RNA complex

Released:
Source organism: Mycobacterium tuberculosis
Primary publication:
Structure of a Mycobacterium tuberculosis NusA-RNA complex.
EMBO J. 24 3576-87 (2005)
PMID: 16193062

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Transcription termination/antitermination protein NusA Chain: A
Molecule details ›
Chain: A
Length: 251 amino acids
Theoretical weight: 27.01 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P9WIV3 (Residues: 105-347; Coverage: 70%)
Gene names: MTCY16B7.01, MTCY24A1.16, Rv2841c, nusA
Sequence domains: NusA-like KH domain
Structure domains:
ribosomal RNA (5'- GAACUCAAUAG -3') Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.51 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: P4122
Unit cell:
a: 71.416Å b: 71.416Å c: 115.219Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.222
Expression systems:
  • Escherichia coli BL21
  • Not provided