2an6

X-ray diffraction
3Å resolution

Protein-peptide complex

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
Non-polymer only tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase SIAH1A Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 191 amino acids
Theoretical weight: 21.54 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P61092 (Residues: 92-282; Coverage: 68%)
Gene name: Siah1a
Structure domains:
peptide from Phyllopod Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 24 amino acids
Theoretical weight: 2.85 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P21
Unit cell:
a: 63.3Å b: 100Å c: 103.4Å
α: 90° β: 104.3° γ: 90°
R-values:
R R work R free
0.241 0.237 0.305
Expression systems:
  • Escherichia coli
  • Not provided