2agh

Solution NMR

Structural basis for cooperative transcription factor binding to the CBP coactivator

Released:

Function and Biology Details

Reactions catalysed:
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone]

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Transcriptional activator Myb Chain: A
Molecule details ›
Chain: A
Length: 25 amino acids
Theoretical weight: 2.95 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06876 (Residues: 291-315; Coverage: 4%)
Gene name: Myb
CREB-binding protein Chain: B
Molecule details ›
Chain: B
Length: 87 amino acids
Theoretical weight: 10.35 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P45481 (Residues: 586-672; Coverage: 4%)
Gene names: Cbp, Crebbp
Sequence domains: KIX domain
Structure domains: Coactivator CBP, KIX domain
MLL cleavage product C180 Chain: C
Molecule details ›
Chain: C
Length: 31 amino acids
Theoretical weight: 3.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q03164 (Residues: 2839-2869; Coverage: 1%)
Gene names: ALL1, CXXC7, HRX, HTRX, KMT2A, MLL, MLL1, TRX1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 9%
Refinement method: DYANA & AMBER
Chemical shifts: BMR6750  
Expression system: Escherichia coli BL21(DE3)