2af7

X-ray diffraction
2.81Å resolution

Crystal structure of the gamma-carboxymuconolactone decarboxylase from Methanobacterium thermoautotrophicum. Northeast Structural Genomics Consortium target TT747.

Released:
Entry authors: Vorobiev SM, Kuzin A, Skarina T, Savchenko A, Semesi A, Arrowsmith C, Edwards A, Montelione GT, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed:
2-carboxy-2,5-dihydro-5-oxofuran-2-acetate = 4,5-dihydro-5-oxofuran-2-acetate + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CMD domain-containing protein Chains: A, B, C, D, E, F, G, H, I
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I
Length: 125 amino acids
Theoretical weight: 14.22 KDa
Source organism: Methanothermobacter thermautotrophicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O26336 (Residues: 1-125; Coverage: 100%)
Gene name: MTH_234
Sequence domains: Carboxymuconolactone decarboxylase family
Structure domains: AhpD-like

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 183.306Å b: 119.109Å c: 73.62Å
α: 90° β: 102.8° γ: 90°
R-values:
R R work R free
0.245 0.245 0.292
Expression system: Escherichia coli