2a41

X-ray diffraction
2.6Å resolution

Ternary complex of the WH2 Domain of WIP with Actin-DNAse I

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
Non-polymer only trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Actin, alpha skeletal muscle Chain: A
Molecule details ›
Chain: A
Length: 375 amino acids
Theoretical weight: 41.88 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:
Deoxyribonuclease-1 Chain: B
Molecule details ›
Chain: B
Length: 260 amino acids
Theoretical weight: 29.09 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00639 (Residues: 23-282; Coverage: 100%)
Gene names: DNASE1, DNL1
Sequence domains: Endonuclease/Exonuclease/phosphatase family
Structure domains: Endonuclease/exonuclease/phosphatase
WAS/WASL-interacting protein family member 1 Chain: C
Molecule details ›
Chain: C
Length: 32 amino acids
Theoretical weight: 3.47 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O43516 (Residues: 29-60; Coverage: 6%)
Gene names: WASPIP, WIP, WIPF1
Sequence domains: WH2 motif

Ligands and Environments


1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P212121
Unit cell:
a: 41.8Å b: 77.07Å c: 222.29Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.16 0.219
Expression system: Not provided