X-ray diffraction
1.8Å resolution

Ternary complex of the WH2 domain of WAVE with Actin-DNAse I


Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products
Biochemical function:
Cellular component:

Structure analysis Details

Assemblies composition:
Non-polymer only trimer (preferred)
Non-polymer only hexamer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Actin, alpha skeletal muscle Chains: A, D
Molecule details ›
Chains: A, D
Length: 375 amino acids
Theoretical weight: 41.88 KDa
Source organism: Oryctolagus cuniculus
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:
Deoxyribonuclease-1 Chains: B, E
Molecule details ›
Chains: B, E
Length: 260 amino acids
Theoretical weight: 29.09 KDa
Source organism: Bos taurus
  • Canonical: P00639 (Residues: 23-282; Coverage: 100%)
Gene names: DNASE1, DNL1
Sequence domains: Endonuclease/Exonuclease/phosphatase family
Structure domains: Endonuclease/exonuclease/phosphatase
Wiskott-Aldrich syndrome protein family member 2 Chains: C, F
Molecule details ›
Chains: C, F
Length: 32 amino acids
Theoretical weight: 3.82 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: Q9Y6W5 (Residues: 433-464; Coverage: 6%)
Gene names: WASF2, WAVE2
Sequence domains: WH2 motif

Ligands and Environments

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 118.719Å b: 41.598Å c: 153.048Å
α: 90° β: 108.79° γ: 90°
R R work R free
0.169 0.166 0.215
Expression system: Not provided