X-ray diffraction
2.08Å resolution

Ternary complex of the WH2 domain of WASP with Actin-DNAse I


Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
Non-polymer only trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Actin, alpha skeletal muscle Chain: A
Molecule details ›
Chain: A
Length: 375 amino acids
Theoretical weight: 41.88 KDa
Source organism: Oryctolagus cuniculus
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:
Deoxyribonuclease-1 Chain: B
Molecule details ›
Chain: B
Length: 260 amino acids
Theoretical weight: 29.09 KDa
Source organism: Bos taurus
  • Canonical: P00639 (Residues: 23-282; Coverage: 100%)
Gene names: DNASE1, DNL1
Sequence domains: Endonuclease/Exonuclease/phosphatase family
Structure domains: Endonuclease/exonuclease/phosphatase
Wiskott-Aldrich syndrome protein Chain: C
Molecule details ›
Chain: C
Length: 29 amino acids
Theoretical weight: 3.02 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: P42768 (Residues: 430-458; Coverage: 6%)
Gene names: IMD2, WAS

Ligands and Environments

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: C2
Unit cell:
a: 153.27Å b: 41.798Å c: 119.418Å
α: 90° β: 108.68° γ: 90°
R R work R free
0.159 0.153 0.21
Expression system: Not provided