› 1zzc

X-ray diffraction
1.8Å resolution

Crystal Structure of CoII HppE in Complex with Tris Buffer

Released:
DOI: 10.2210/pdb1zzc/pdb
PDB entry 1zzc coloured by chain, front view.
PDB entry 1zzc coloured by chain, side view.
PDB entry 1zzc coloured by chain, top view.
Source organism: Streptomyces wedmorensis
Primary publication:
Structural insight into antibiotic fosfomycin biosynthesis by a mononuclear iron enzyme.
Higgins LJ, Yan F, Liu P, Liu HW, Drennan CL
Nature 437 838-44 (2005)
PMID: 16015285

Function and Biology Details

Reaction catalysed: 
(S)-2-hydroxypropylphosphonate + H(2)O(2) = (1R,2S)-1,2-epoxypropylphosphonate + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176324 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(S)-2-hydroxypropylphosphonic acid epoxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 198 amino acids
Theoretical weight: 21.36 KDa
Source organism: Streptomyces wedmorensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q56185 (Residues: 1-198; Coverage: 100%)
Gene names: fom4, hppE
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CO 2 x CO
Ligand TRS 2 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 8-BM
Spacegroup: P6522
Unit cell:
a: 86.36Å b: 86.36Å c: 220.17Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.225 0.242
Expression system: Escherichia coli BL21(DE3)

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Citations

15 review citations

Structural studies on 2-oxoglutarate oxygenases and related double-stranded beta-helix fold proteins.
Clifton et al. (2006)
14 more

2 mentions without citation

Structural basis of regiospecificity of a mononuclear iron enzyme in antibiotic fosfomycin biosynthesis.
Yun et al. (2011)
1 more