1zvv

X-ray diffraction
2.98Å resolution

Crystal structure of a ccpa-crh-dna complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (3 distinct):
Catabolite control protein A Chains: A, B, G
Molecule details ›
Chains: A, B, G
Length: 332 amino acids
Theoretical weight: 36.67 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25144 (Residues: 3-333; Coverage: 99%)
Gene names: BSU29740, alsA, amyR, ccpA, graR
Sequence domains:
Structure domains:
HPr-like protein Crh Chains: J, P, W
Molecule details ›
Chains: J, P, W
Length: 85 amino acids
Theoretical weight: 9.46 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O06976 (Residues: 1-85; Coverage: 100%)
Gene names: BSU34740, crh, yvcM
Sequence domains: PTS HPr component phosphorylation site
Structure domains: HPr-like
DNA recognition strand CRE Chains: O, R, T
Molecule details ›
Chains: O, R, T
Length: 16 nucleotides
Theoretical weight: 4.91 KDa

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2
Unit cell:
a: 83.69Å b: 158.1Å c: 125.47Å
α: 90° β: 100.73° γ: 90°
R-values:
R R work R free
0.235 0.235 0.298
Expression system: Escherichia coli BL21(DE3)