› 1zvd

X-ray diffraction
2.1Å resolution

Regulation of Smurf2 Ubiquitin Ligase Activity by Anchoring the E2 to the HECT domain

Released:
DOI: 10.2210/pdb1zvd/pdb
PDB entry 1zvd coloured by chain, front view.
PDB entry 1zvd coloured by chain, side view.
PDB entry 1zvd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Regulation of Smurf2 ubiquitin ligase activity by anchoring the E2 to the HECT domain.
Ogunjimi AA, Briant DJ, Pece-Barbara N, Le Roy C, Di Guglielmo GM, Kavsak P, Rasmussen RK, Seet BT, Sicheri F, Wrana JL
Mol Cell 19 297-308 (2005)
PMID: 16061177

Function and Biology Details

Reaction catalysed: 
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190727 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase SMURF2 Chain: A
Molecule details ›
Chain: A
Length: 380 amino acids
Theoretical weight: 44.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HAU4 (Residues: 369-748; Coverage: 51%)
Gene name: SMURF2
Sequence domains: HECT-domain (ubiquitin-transferase)
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand PO4 1 x PO4
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: P212121
Unit cell:
a: 51.359Å b: 70.509Å c: 152.351Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.202 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

58 review citations

Modification of proteins by ubiquitin and ubiquitin-like proteins.
Kerscher et al. (2006)
57 more

16 mentions without citation

Structural insights into the catalysis and regulation of E3 ubiquitin ligases.
Buetow et al. (2016)
15 more