PDBe 1zsy

X-ray diffraction
1.75Å resolution

The structure of human mitochondrial 2-enoyl thioester reductase (CGI-63)

Released:
Source organism: Homo sapiens
Entry authors: Lukacik P, Shafqat N, Kavanagh KL, Johansson C, Smee C, Edwards A, Arrowsmith C, Sundstrom M, von Delft F, Oppermann U, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed:
An acyl-[acyl-carrier protein] + NADP(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enoyl-[acyl-carrier-protein] reductase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 357 amino acids
Theoretical weight: 38.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BV79 (Residues: 40-373; Coverage: 90%)
Gene names: CGI-63, MECR, NBRF1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: I222
Unit cell:
a: 74.19Å b: 117.184Å c: 127.372Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.17 0.195
Expression system: Escherichia coli BL21(DE3)