Solution NMR

beta PIX-SH3 complexed with an atypical peptide from alpha-PAK


Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rho guanine nucleotide exchange factor 7 Chain: A
Molecule details ›
Chain: A
Length: 65 amino acids
Theoretical weight: 7.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q14155 (Residues: 179-243; Coverage: 8%)
Gene names: ARHGEF7, COOL1, KIAA0142, Nbla10314, P85SPR, PAK3BP, PIXB
Sequence domains: Variant SH3 domain
Structure domains: SH3 Domains
Serine/threonine-protein kinase PAK 1 Chain: B
Molecule details ›
Chain: B
Length: 22 amino acids
Theoretical weight: 2.42 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: Q13153 (Residues: 183-204; Coverage: 4%)
Gene name: PAK1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simluated annealing with torsion angle dynamics followed by Cartesian dynamics cooling
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided