1zre

X-ray diffraction
2.8Å resolution

4 crystal structures of CAP-DNA with all base-pair substitutions at position 6, CAP-[6G;17C]ICAP38 DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
cAMP-activated global transcriptional regulator CRP Chains: A, B
Molecule details ›
Chains: A, B
Length: 209 amino acids
Theoretical weight: 23.54 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ACJ8 (Residues: 2-210; Coverage: 100%)
Gene names: JW5702, b3357, cap, crp, csm
Sequence domains:
Structure domains:
5'-D(*AP*TP*TP*TP*CP*GP*AP*AP*AP*AP*AP*TP*GP*GP*GP*AP*T)-3' Chains: W, Y
Molecule details ›
Chains: W, Y
Length: 17 nucleotides
Theoretical weight: 5.27 KDa
Source organism: Escherichia coli
Expression system: Not provided
5'-D(*CP*TP*AP*GP*AP*TP*CP*CP*CP*AP*TP*TP*TP*TP*TP*CP*GP*AP*AP*AP*T)-3' Chains: X, Z
Molecule details ›
Chains: X, Z
Length: 21 nucleotides
Theoretical weight: 6.37 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 61.352Å b: 75.566Å c: 179.979Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.285 0.233 0.283
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided