› 1zp7

X-ray diffraction
2.25Å resolution

The structure of Bacillus subtilis RecU Holliday junction resolvase and its role in substrate selection and sequence specific cleavage.

Released:
DOI: 10.2210/pdb1zp7/pdb
PDB entry 1zp7 coloured by chain, front view.
PDB entry 1zp7 coloured by chain, side view.
PDB entry 1zp7 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
The structure of Bacillus subtilis RecU Holliday junction resolvase and its role in substrate selection and sequence-specific cleavage.
McGregor N, Ayora S, Sedelnikova S, Carrasco B, Alonso JC, Thaw P, Rafferty J
Structure 13 1341-51 (2005)
PMID: 16154091

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153951 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Holliday junction resolvase RecU Chains: A, B
Molecule details ›
Chains: A, B
Length: 206 amino acids
Theoretical weight: 24 KDa
Source organism: Bacillus subtilis
UniProt:
  • Canonical: P39792 (Residues: 1-206; Coverage: 100%)
Gene names: BSU22310, prfA, recU, yppB
Sequence domains: Recombination protein U
Structure domains: Trna Endonuclease; Chain: A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: P65
Unit cell:
a: 74.34Å b: 74.34Å c: 153.466Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.217 0.269

Quick links

Citations

9 review citations

Double-strand break repair in bacteria: a view from Bacillus subtilis.
Ayora et al. (2011)
8 more

7 mentions without citation

DNA repair and genome maintenance in Bacillus subtilis.
Lenhart et al. (2012)
6 more