Solution NMR

Solution structure of a human ubiquitin-like domain in SF3A1

Source organism: Homo sapiens
Entry authors: Lukin JA, Dhe-Paganon S, Guido V, Lemak A, Avvakumov GV, Xue S, Newman EM, Mackenzie F, Sundstrom M, Edwards A, Arrowsmith CH, Structural Genomics Consortium (SGC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Splicing factor 3A subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 86 amino acids
Theoretical weight: 9.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: Q15459 (Residues: 704-789; Coverage: 11%)
Gene names: SAP114, SF3A1
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: 100 structures were calculated by CYANA 2.0 using 2667 NOE-derived distance constraints, 121 dihedral angle constraints, and 50 distance constraints from hydrogen bonds. The 20 structures with lowest target functions were subjected to refinement with CNS 1.1.
Chemical shifts: BMR6631  
Expression system: Escherichia coli