1zhh

X-ray diffraction
1.94Å resolution

Crystal Structure of the Apo Form of Vibrio Harveyi LUXP Complexed with the Periplasmic Domain of LUXQ

Released:

Function and Biology Details

Reaction catalysed:
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Autoinducer 2-binding periplasmic protein LuxP Chain: A
Molecule details ›
Chain: A
Length: 344 amino acids
Theoretical weight: 39.29 KDa
Source organism: Vibrio harveyi
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P54300 (Residues: 22-365; Coverage: 100%)
Gene name: luxP
Sequence domains: Periplasmic binding protein domain
Structure domains: Rossmann fold
Autoinducer 2 sensor kinase/phosphatase LuxQ Chain: B
Molecule details ›
Chain: B
Length: 242 amino acids
Theoretical weight: 27.41 KDa
Source organism: Vibrio harveyi
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P54302 (Residues: 39-278; Coverage: 28%)
Gene name: luxQ
Sequence domains: LuxQ, periplasmic
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P65
Unit cell:
a: 128.468Å b: 128.468Å c: 95.671Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.24 0.206 0.222
Expression system: Escherichia coli BL21