1zbq

X-ray diffraction
2.19Å resolution

Crystal Structure Of Human 17-Beta-Hydroxysteroid Dehydrogenase Type 4 In Complex With NAD

Released:
Source organism: Homo sapiens
Entry authors: Lukacik P, Shafqat N, Kavanagh K, Bray J, von Delft F, Edwards A, Arrowsmith C, Sundstrom M, Oppermann U, Structural Genomics Consortium (SGC)

Function and Biology Details

Reactions catalysed:
(R)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH
(3R)-3-hydroxyacyl-CoA = (2E)-2-enoyl-CoA + H(2)O 
(24R,25R)-3-alpha,7-alpha,12-alpha,24-tetrahydroxy-5-beta-cholestanoyl-CoA = (24E)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA + H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(3R)-hydroxyacyl-CoA dehydrogenase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 327 amino acids
Theoretical weight: 35.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P51659 (Residues: 1-304; Coverage: 41%)
Gene names: EDH17B4, HSD17B4, SDR8C1
Sequence domains: short chain dehydrogenase
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P1
Unit cell:
a: 50.826Å b: 50.771Å c: 183.775Å
α: 87.52° β: 87.07° γ: 70.75°
R-values:
R R work R free
0.19 0.188 0.236
Expression system: Escherichia coli BL21(DE3)