› 1z16

X-ray diffraction
1.72Å resolution

Crystal structure analysis of periplasmic Leu/Ile/Val-binding protein with bound leucine

Released:
DOI: 10.2210/pdb1z16/pdb
PDB 1z16 coloured by chain and viewed from the front.
PDB 1z16 coloured by chain and viewed from the side.
PDB 1z16 coloured by chain and viewed from the top.
Source organism: Escherichia coli K-12
Primary publication:
Ligand-free and -bound structures of the binding protein (LivJ) of the Escherichia coli ABC leucine/isoleucine/valine transport system: trajectory and dynamics of the interdomain rotation and ligand specificity.
Trakhanov S, Vyas NK, Luecke H, Kristensen DM, Ma J, Quiocho FA
Biochemistry 44 6597-608 (2005)
PMID: 15850393

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leu/Ile/Val-binding protein Chain: A
Molecule details ›
Chain: A
Length: 344 amino acids
Theoretical weight: 36.81 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0AD96 (Residues: 24-367; Coverage: 100%)
Gene names: JW3425, b3460, livJ
Sequence domains: Periplasmic binding protein
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand CD 1 x CD
Ligand LEU 1 x LEU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P64
Unit cell:
a: 87.65Å b: 87.65Å c: 94.04Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.175 0.21

Quick links

Citations

9 review citations

Normal Mode Analysis as a Routine Part of a Structural Investigation.
Bauer et al. (2019)
8 more

6 mentions without citation

A conserved mechanism of GABA binding and antagonism is revealed by structure-function analysis of the periplasmic binding protein Atu2422 in Agrobacterium tumefaciens.
Planamente et al. (2010)
5 more