› 1yy5

X-ray diffraction
2.3Å resolution

Crystal structure of Fms1, a polyamine oxidase from Yeast

Released:
DOI: 10.2210/pdb1yy5/pdb
PDB entry 1yy5 coloured by chain, front view.
PDB entry 1yy5 coloured by chain, side view.
PDB entry 1yy5 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structures of Fms1 and its complex with spermine reveal substrate specificity.
Huang Q, Liu Q, Hao Q
J Mol Biol 348 951-9 (2005)
PMID: 15843025

Function and Biology Details

Reaction catalysed: 
N(1)-acetylspermidine + O(2) + H(2)O = putrescine + 3-acetamidopropanal + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156155 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyamine oxidase FMS1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 513 amino acids
Theoretical weight: 58.53 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P50264 (Residues: 1-508; Coverage: 100%)
Gene names: FMS1, YM9711.09, YMR020W
Sequence domains: Flavin containing amine oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: C2221
Unit cell:
a: 102.711Å b: 215.365Å c: 118.157Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.249 0.249 0.29
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Elucidating biosynthetic pathways for vitamins and cofactors.
Webb et al. (2007)
4 more

3 mentions without citation

Oxidation of amines by flavoproteins.
Fitzpatrick PF. (2010)
2 more