1ytb

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF A YEAST TBP/TATA-BOX COMPLEX

Released:
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of a yeast TBP/TATA-box complex.
Nature 365 512-20 (1993)
PMID: 8413604

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
TATA-box-binding protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 180 amino acids
Theoretical weight: 20.12 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P13393 (Residues: 61-240; Coverage: 75%)
Gene names: BTF1, SPT15, TBP1, YER148W
Sequence domains: Transcription factor TFIID (or TATA-binding protein, TBP)
Structure domains: TATA-Binding Protein
DNA (29MER) Chains: C, D
Molecule details ›
Chains: C, D
Length: 29 nucleotides
Theoretical weight: 8.99 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P43
Unit cell:
a: 100.77Å b: 100.77Å c: 66.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.201 0.261
Expression systems:
  • Escherichia coli
  • Not provided