› 1ysj

X-ray diffraction
2.4Å resolution

Crystal Structure of Bacillus Subtilis YXEP Protein (APC1829), a Dinuclear Metal Binding Peptidase from M20 Family

Released:
DOI: 10.2210/pdb1ysj/pdb
PDB entry 1ysj coloured by chain, front view.
PDB entry 1ysj coloured by chain, side view.
PDB entry 1ysj coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Minasov G, Shuvalova L, Brunzelle JS, Collart FR, Anderson WF, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157131 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetylcysteine deacetylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 404 amino acids
Theoretical weight: 44.84 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P54955 (Residues: 1-380; Coverage: 100%)
Gene names: BSU39470, LP9H, scmP, sndB, yxeP
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NI 4 x NI
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 32-ID
Spacegroup: C2
Unit cell:
a: 233.854Å b: 42.157Å c: 75.449Å
α: 90° β: 106.713° γ: 90°
R-values:
R R work R free
0.185 0.182 0.247
Expression system: Escherichia coli BL21(DE3)

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