1yrv

X-ray diffraction
2.18Å resolution

Novel Ubiquitin-Conjugating Enzyme

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin-conjugating enzyme E2 U Chain: A
Molecule details ›
Chain: A
Length: 169 amino acids
Theoretical weight: 19.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5VVX9 (Residues: 1-150; Coverage: 47%)
Gene name: UBE2U
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E
Spacegroup: I222
Unit cell:
a: 47.968Å b: 74.009Å c: 119.733Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.244 0.294
Expression system: Escherichia coli BL21(DE3)