1yns

X-ray diffraction
1.7Å resolution

Crystal Structure Of Human Enolase-phosphatase E1 and its complex with a substrate analog

Released:

Function and Biology Details

Reaction catalysed:
(1a) 5-(methylsulfanyl)-2,3-dioxopentyl phosphate = 2-hydroxy-5-(methylsulfanyl)-3-oxopent-1-enyl phosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enolase-phosphatase E1 Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 28.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UHY7 (Residues: 1-261; Coverage: 100%)
Gene names: ENOPH1, MASA, MSTP145
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P212121
Unit cell:
a: 54.017Å b: 57.546Å c: 87.318Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.256 0.225 0.234
Expression system: Escherichia coli BL21(DE3)