1yj9

X-ray diffraction
2.8Å resolution

Crystal Structure Of The Mutant 50S Ribosomal Subunit Of Haloarcula Marismortui Containing a three residue deletion in L22

Released:

Function and Biology Details

Reactions catalysed:
Hydrolysis of proteins in presence of ATP.
4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O
4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP
5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine
ATP + a protein = ADP + a phosphoprotein
Shikimate + NADP(+) = 3-dehydroshikimate + NADPH
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
Tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein]
Random hydrolysis of (1->6)-alpha-D-mannosidic linkages in unbranched (1->6)-mannans
N(6)-prenyladenine + acceptor + H(2)O = adenine + 3-methylbut-2-enal + reduced acceptor
2 superoxide + 2 H(+) = O(2) + H(2)O(2)
ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)
TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
NTP + H(2)O = NDP + phosphate
H(2) + 2 ferricytochrome c3 = 2 H(+) + 2 ferrocytochrome c3
S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O
H(2) + A = AH(2)
2 H(2)O(2) = O(2) + 2 H(2)O
L-histidinol phosphate + H(2)O = L-histidinol + phosphate
Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends
S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
A 2'-deoxyribonucleoside 5'-monophosphate + H(2)O = a 2'-deoxyribonucleoside + phosphate
L-arginine + 2-oxoglutarate + O(2) = (3S)-3-hydroxy-L-arginine + succinate + CO(2)
Methyl-CoM + CoB = CoM-S-S-CoB + methane
Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H(2)O
A purine nucleoside + H(2)O = D-ribose + a purine base
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
dUTP + H(2)O = dUMP + diphosphate
7,8-dihydroneopterin 3'-triphosphate = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
H(2) + oxidized coenzyme F420 = reduced coenzyme F420
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid
Chorismate = prephenate
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
Inosine 5'-phosphate + NAD(+) + H(2)O = xanthosine 5'-phosphate + NADH
Cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose
A beta-lactam + H(2)O = a substituted beta-amino acid
GDP-alpha-D-mannose = GDP-4-dehydro-alpha-D-rhamnose + H(2)O
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Peptidylproline (omega=180) = peptidylproline (omega=0)
RH + Cl(-) + H(2)O(2) = RCl + 2 H(2)O
Cyclomaltodextrin + H(2)O = linear maltodextrin
N(4)-acetyl-L-2,4-diaminobutanoate = L-ectoine + H(2)O
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
(1a) cyanate + HCO(3)(-) + H(+) = carbamate + CO(2)
ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly)
IMP + H(2)O = inosine + phosphate
ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)
ATP + H(2)O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide
ATP + H(2)O = ADP + phosphate
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Acetyl-CoA + maltose = CoA + acetyl-maltose
An anilide + H(2)O = a carboxylate + aniline
L-lysine + NADPH + O(2) = N(6)-hydroxy-L-lysine + NADP(+) + H(2)O
CoB + CoM + methanophenazine = CoM-S-S-CoB + dihydromethanophenazine
An aliphatic amide = a nitrile + H(2)O
A carboxylic ester + H(2)O = an alcohol + a carboxylate
(R)-S-lactoylglutathione = glutathione + methylglyoxal
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
H(2)CO(3) = CO(2) + H(2)O
Hydrolysis of Xaa-|-Pro dipeptides. Also acts on aminoacyl-hydroxyproline analogs. No action on Pro-|-Pro.
(S)-dihydroorotate + fumarate = orotate + succinate
An acyl-[acyl-carrier protein] + NAD(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH
6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H(2)O = D-glucose + D-glucose 6-phosphate
R-CHOH-R' + NADP(+) = R-CO-R' + NADPH
ATP + pyruvate = ADP + phosphoenolpyruvate
ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-Casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
UDP-N-acetyl-alpha-D-galactosamine + [protein]-L-serine = UDP + [protein]-3-O-(N-acetyl-alpha-D-galactosaminyl)-L-serine
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
(1a) Man(9)GlcNAc(2)-[protein] + H(2)O = Man(8)GlcNAc(2)-[protein] (isomer 8A(1,2,3)B(1,2)) + beta-D-mannopyranose
Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate
5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate
Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides
Quinol + 2 ferricytochrome c(Side 2) = quinone + 2 ferrocytochrome c(Side 2) + 2 H(+)(Side 2)
5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(In) = 5,6,7,8-tetrahydromethanopterin + 2-(methylsulfanyl)ethane-1-sulfonate + 2 Na(+)(Out)
(S)-2-hydroxy acid + O(2) = 2-oxo acid + H(2)O(2)
Chloride + O(2) = chlorite
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
Xyloglucan + H(2)O = xyloglucan oligosaccharides
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Sphingomyelin + H(2)O = N-acylsphingosine + phosphocholine
O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate
2'-(5-triphosphoribosyl)-3'-dephospho-CoA + citrate lyase apo-[acyl-carrier protein] = citrate lyase holo-[acyl-carrier protein] + diphosphate
Hydrolysis of terminal, non-reducing branched (1->3)-alpha-D-galactosidic residues, producing free D-galactose
ATP + kanamycin = ADP + kanamycin 3'-phosphate
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin
Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate
(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H(2)O
N-substituted aminoacyl-tRNA + H(2)O = N-substituted amino acid + tRNA
Urea + H(2)O = CO(2) + 2 NH(3)
Produces alpha-D-fructofuranose beta-D-fructofuranose 1,2':2,1'-dianhydride (DFA I) by successively eliminating the diminishing (2->1)-beta-D-fructan (inulin) chain from the terminal D-fructosyl-D-fructosyl disaccharide.
Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate
Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds
2 phenolic donor + H(2)O(2) = 2 phenoxyl radical of the donor + 2 H(2)O
(R)-3-hydroxybutanoate + NAD(+) = acetoacetate + NADH
Beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole = nicotinate + alpha-ribazole 5'-phosphate
ATP + glycerol = ADP + sn-glycerol 3-phosphate
Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain
D-fructose 6-phosphate + phosphate = acetyl phosphate + D-erythrose 4-phosphate + H(2)O
Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Thioredoxin + NADP(+) = thioredoxin disulfide + NADPH
L-rhamnonate = 2-dehydro-3-deoxy-L-rhamnonate + H(2)O
(9Z)-(13S)-12,13-epoxyoctadeca-9,11,15-trienoate = (15Z)-12-oxophyto-10,15-dienoate
2-dehydro-3-deoxy-6-phosphate-D-gluconate = pyruvate + D-glyceraldehyde 3-phosphate
D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate
D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH
L-histidinol + H(2)O + 2 NAD(+) = L-histidine + 2 NADH
ATP + acetyl-CoA + HCO(3)(-) = ADP + phosphate + malonyl-CoA
ATP + propanoyl-CoA + HCO(3)(-) = ADP + phosphate + (S)-methylmalonyl-CoA
Diphosphate + H(2)O = 2 phosphate
ATP + (R)-5-diphosphomevalonate = ADP + phosphate + isopentenyl diphosphate + CO(2)
GTP = 3',5'-cyclic GMP + diphosphate
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids
ATP + D-ribose = ADP + D-ribose 5-phosphate
N-carbamoylputrescine + H(2)O = putrescine + CO(2) + NH(3)
Prephenate + NADP(+) = 4-hydroxyphenylpyruvate + CO(2) + NADPH
CoA-(4'-phosphopantetheine) + apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier-protein]
L-erythro-3,5-diaminohexanoate + H(2)O + NAD(+) = (S)-5-amino-3-oxohexanoate + NH(3) + NADH
D-glyceraldehyde 3-phosphate = glycerone phosphate
An aldehyde + H(2)O + 2 oxidized ferredoxin = a carboxylate + 2 H(+) + 2 reduced ferredoxin
S-(hydroxymethyl)glutathione + NAD(P)(+) = S-formylglutathione + NAD(P)H
2-deoxy-D-ribose 5-phosphate = D-glyceraldehyde 3-phosphate + acetaldehyde
ATP + 3'-dephospho-CoA = ADP + CoA
A glutathione-S-conjugate + H(2)O = an (L-cysteinylglycine)-S-conjugate + L-glutamate
2-alpha-D-glucosyl-D-glucose + phosphate = D-glucose + beta-D-glucose 1-phosphate
L-alanine + H(2)O + NAD(+) = pyruvate + NH(3) + NADH
4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate
Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.
Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans
ATP-dependent breakage, passage and rejoining of double-stranded DNA
RH + [reduced NADPH--hemoprotein reductase] + O(2) = ROH + [oxidized NADPH--hemoprotein reductase] + H(2)O
A (3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-N-acetyl-alpha-D-glucosamine = an [acyl-carrier-protein] + a UDP-3-O-(3-hydroxyacylyl)-N-acetyl-alpha-D-glucosamine
Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate
A monocarboxylic acid amide + H(2)O = a monocarboxylate + NH(3)
L-erythrulose 1-phosphate = D-erythrulose 4-phosphate
S-adenosyl-L-homocysteine + H(2)O = L-homocysteine + adenosine
D-glucose + NAD(P)(+) = D-glucono-1,5-lactone + NAD(P)H
Acetyl-CoA + H(2)O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Phosphoenolpyruvate + D-erythrose 4-phosphate + H(2)O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate 
Formate + NAD(+) = CO(2) + NADH
N-acetyl-O-acetylneuraminate + H(2)O = N-acetylneuraminate + acetate
ATP + H(2)O + cellular protein(Side 1) = ADP + phosphate + cellular protein(Side 2)
L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate
Succinate + a quinone = fumarate + a quinol
(S)-2-haloacid + H(2)O = (R)-2-hydroxyacid + halide
An alpha-L-fucoside + H(2)O = L-fucose + an alcohol
Catechol + O(2) = cis,cis-muconate
3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO(2)
1-haloalkane + H(2)O = a primary alcohol + halide
O-phospho-L(or D)-serine + H(2)O = L(or D)-serine + phosphate
L-cystathionine + H(2)O = L-homocysteine + NH(3) + pyruvate
(1a) L-cystathionine = L-cysteine + 2-aminobut-2-enoate
N-carbamoyl-L-2-amino acid (a 2-ureido carboxylate) + H(2)O = L-2-amino acid + NH(3) + CO(2)
(R)-2-haloacid + H(2)O = (S)-2-hydroxyacid + halide
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose
Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains
(S)-malate = fumarate + H(2)O
ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate
A (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid
An aromatic primary alcohol + O(2) = an aromatic aldehyde + H(2)O(2)
(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
L-glutamate + H(2)O + NADP(+) = 2-oxoglutarate + NH(3) + NADPH
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
A C(21)-steroid + [reduced NADPH--hemoprotein reductase] + O(2) = a 17-alpha-hydroxy-C(21)-steroid + [oxidized NADPH--hemoprotein reductase] + H(2)O
UDP-alpha-D-glucose = UDP-alpha-D-galactose
S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol
L-asparagine + H(2)O = L-aspartate + NH(3)
(1a) (2R,3S)-3-isopropylmalate + NAD(+) = (2S)-2-isopropyl-3-oxosuccinate + NADH
(Ribonucleotide)(n)-2',3'-cyclic phosphate + H(2)O = (ribonucleotide)(n)-2'-phosphate
ATP + biotin + [biotin carboxyl-carrier protein]-L-lysine = AMP + diphosphate + [biotin carboxyl-carrier protein]-N(6)-biotinyl-L-lysine
dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-alpha-D-glucose
RX + glutathione = HX + R-S-glutathione
Similar to that of papain.
Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans
(R)-mevalonate + CoA + 2 NADP(+) = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH
(S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH
Taurine + 2-oxoglutarate + O(2) = sulfite + aminoacetaldehyde + succinate + CO(2)
UDP-N-acetyl-alpha-D-mannosamine + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = UDP + N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
(1a) N(6)-(3-methylbut-2-en-1-yl)-adenine(37) in tRNA + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced electron acceptor = N(6)-(3-methylbut-2-en-1-yl)-2-thioadenine(37) in tRNA + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor
Alpha-D-glucosamine 6-phosphate + H(2)O = D-fructose 6-phosphate + NH(3)
ATP + pyridoxal = ADP + pyridoxal 5'-phosphate
A pyrimidine nucleoside + H(2)O = D-ribose + a pyrimidine base
5'-deoxyadenosine + H(2)O = 5-deoxy-D-ribose + adenine
2-iminobutanoate + H(2)O = 2-oxobutanoate + NH(3)
A 3-beta-hydroxy-Delta(5)-steroid + NAD(+) = a 3-oxo-Delta(5)-steroid + NADH
UDP-N-acetyl-alpha-D-glucosamine = UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H(2)O
H(2) + 5,10-methenyltetrahydromethanopterin = H(+) + 5,10-methylenetetrahydromethanopterin
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains
17-alpha-hydroxypregnenolone + [reduced NADPH--hemoprotein reductase] + O(2) = 3-beta-hydroxyandrost-5-en-17-one + acetate + [oxidized NADPH--hemoprotein reductase] + H(2)O
Oxalyl-CoA = formyl-CoA + CO(2)
Aceneneuramate = N-acetyl-D-mannosamine + pyruvate
(S)-3-hydroxybutanoyl-CoA + NADP(+) = 3-acetoacetyl-CoA + NADPH
N,N-dimethylformamide + H(2)O = dimethylamine + formate
D-glucosyl-N-acylsphingosine + H(2)O = D-glucose + N-acylsphingosine
Formylmethanofuran + 5,6,7,8-tetrahydromethanopterin = methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin
ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)
L-arginine + H(2)O = L-ornithine + urea
4-coumarate = 4-vinylphenol + CO(2)
D-gluconate + NAD(P)(+) = 5-dehydro-D-gluconate + NAD(P)H
2 glutathione + ROOH = glutathione disulfide + H(2)O + ROH
2 3-phospho-D-glycerate + 2 H(+) = D-ribulose 1,5-bisphosphate + CO(2) + H(2)O
[Dinitrogen reductase]-N(omega)-alpha-(ADP-D-ribosyl)-L-arginine = ADP-D-ribose + [dinitrogen reductase]-L-arginine
NADH + ubiquinone + 5 H(+)(Side 1) = NAD(+) + ubiquinol + 4 H(+)(Side 2)
Hg + NADP(+) + H(+) = Hg(2+) + NADPH
ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn)
Protein dithiol + NAD(P)(+) = protein disulfide + NAD(P)H
ATP + deamido-NAD(+) + NH(3) = AMP + diphosphate + NAD(+)
(2S)-flavan-4-ol + NADP(+) = (2S)-flavanone + NADPH
ATP + L-glutamate + NH(3) = ADP + phosphate + L-glutamine
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate = (S)-allantoin + CO(2)
L-glutamate 5-semialdehyde + NAD(+) + H(2)O = L-glutamate + NADH
Cutin + H(2)O = cutin monomers
ATP + 6-hydroxymethyl-7,8-dihydropterin = AMP + 6-hydroxymethyl-7,8-dihydropterin diphosphate
L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate
5-hydroxyisourate + H(2)O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate
(+)-trans-carveol + NAD(+) = (+)-(S)-carvone + NADH
Betaine aldehyde + NAD(+) + H(2)O = betaine + NADH
3-dehydroquinate = 3-dehydroshikimate + H(2)O
2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O
ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = ATP + 5-phospho-alpha-D-ribose 1-diphosphate
3-phospho-D-glycerate + NAD(+) = 3-phosphonooxypyruvate + NADH
(1a) L-tyrosine + 1/2 O(2) = L-dopa
(1a) S-adenosyl-L-methionine + [protein]-L-arginine = S-adenosyl-L-homocysteine + [protein]-N(omega)-methyl-L-arginine
dTDP-beta-L-rhamnose + NADP(+) = dTDP-4-dehydro-beta-L-rhamnose + NADPH
ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)
ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H(2)O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate
Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
6-hydroxymethyl-7,8-dihydropterin diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate
10-formyltetrahydrofolate + H(2)O = formate + tetrahydrofolate
Acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an N-acetyldiamine
(R)-3-hydroxyacyl-CoA + NADP(+) = 3-oxoacyl-CoA + NADPH
6-phospho-D-gluconate + NADP(+) = D-ribulose 5-phosphate + CO(2) + NADPH
(1a) isocitrate + NADP(+) = 2-oxalosuccinate + NADPH
Beta-D-ribopyranose = beta-D-ribofuranose
Sucrose + (6)-beta-D-fructofuranosyl-(2->)(n) alpha-D-glucopyranoside = glucose + (6)-beta-D-fructofuranosyl-(2->)(n+1) alpha-D-glucopyranoside
D-ribulose 5-phosphate = D-xylulose 5-phosphate
An acyl-[acyl-carrier protein] + NADP(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH
7,8-dihydroneopterin 3'-triphosphate + H(2)O = 6-carboxy-5,6,7,8-tetrahydropterin + acetaldehyde + triphosphate
ATP + D-glucose = ADP + D-glucose 6-phosphate
ATP + shikimate = ADP + shikimate 3-phosphate
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO(2)
L-fucopyranose = L-fuculose
(2R,3S)-2-methylmalyl-CoA = propanoyl-CoA + glyoxylate
3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate = urocanate + H(2)O
2 bilirubin + O(2) = 2 biliverdin + 2 H(2)O
L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol
D-allothreonine = glycine + acetaldehyde
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Thioredoxin + ROOH = thioredoxin disulfide + H(2)O + ROH
Formyl-L-methionyl peptide + H(2)O = formate + methionyl peptide
3-deoxy-D-manno-octulosonate 8-phosphate + H(2)O = 3-deoxy-D-manno-octulosonate + phosphate
Autocatalytic release of the core protein from the N-terminus of the togavirus structural polyprotein by hydrolysis of a -Trp-|-Ser- bond.
Autocatalytically cleaves itself from the polyprotein of the foot-and-mouth disease virus by hydrolysis of a Lys-|-Gly bond, but then cleaves host cell initiation factor eIF-4G at bonds -Gly-|-Arg- and -Lys-|-Arg-.
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO(2)
Choline = trimethylamine + acetaldehyde
ADP-alpha-D-glucose + D-glucose 6-phosphate = ADP + alpha,alpha-trehalose 6-phosphate
(R)-pantoate + NADP(+) = 2-dehydropantoate + NADPH
Formamide + H(2)O = formate + NH(3)
ATP + [biotin carboxyl-carrier protein]-biotin-N(6)-L-lysine + hydrogencarbonate- = ADP + phosphate + [biotin carboxyl-carrier protein]-carboxybiotin-N(6)-L-lysine
Hydrolysis of the -Gly-|-Gly- bond in the pentaglycine inter-peptide link joining staphylococcal cell wall peptidoglycans.
Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate = 3-dehydroquinate + phosphate
L-aspartate 4-semialdehyde + phosphate + NADP(+) = L-4-aspartyl phosphate + NADPH
UDP-3-O-((3R)-3-hydroxyacyl)-N-acetyl-alpha-D-glucosamine + H(2)O = UDP-3-O-((3R)-3-hydroxyacyl)-alpha-D-glucosamine + acetate
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA 
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H(2)O
Cyclic pyranopterin phosphate + 2 [molybdopterin-synthase sulfur-carrier protein]-Gly-NH-CH(2)-C(O)SH + H(2)O = molybdopterin + 2 [molybdopterin-synthase sulfur-carrier protein]
(1a) 2 Fe(2+) + O(2) + 4 H(2)O = 2 (FeO(OH)) + 4 H(+) + H(2)O(2)
L-serine + L-homocysteine = L-cystathionine + H(2)O
L-alanine = D-alanine
Protein N(6)-(dihydrolipoyl)lysine + NAD(+) = protein N(6)-(lipoyl)lysine + NADH
Putrescine + O(2) + H(2)O = 4-aminobutanal + NH(3) + H(2)O(2)
(1a) S-adenosyl-L-methionine + L-histidine = S-adenosyl-L-homocysteine + N(alpha)-methyl-L-histidine
L-carnitine + NAD(P)H + O(2) = (3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NAD(P)(+) + H(2)O
L-alanine + 3-oxopropanoate = pyruvate + beta-alanine
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD(+) = 2-methylacetoacetyl-CoA + NADH
An aldehyde + NAD(+) + H(2)O = a carboxylate + NADH
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H(2)O
ATP + (L-Asp(4-L-Arg))(n) + L-Asp = ADP + phosphate + (L-Asp(4-L-Arg))(n)-L-Asp
ATP + acetate = ADP + acetyl phosphate
NADPH + n oxidized hemoprotein = NADP(+) + n reduced hemoprotein
Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini
sn-glycerol 3-phosphate + NAD(P)(+) = glycerone phosphate + NAD(P)H
FAD + [protein]-L-threonine = [protein]-FMN-L-threonine + AMP
[Amino group carrier protein]-C-terminal-gamma-(L-lysyl)-L-glutamate + H(2)O = [amino group carrier protein]-C-terminal-L-glutamate + L-lysine
Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan
(S)-malate + NAD(+) = oxaloacetate + NADH
N-acetyl-alpha-D-glucosamine 1-phosphate = N-acetyl-D-glucosamine 6-phosphate
ADP + phosphate + acetyl-CoA + oxaloacetate = ATP + citrate + CoA
ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate = ADP + 1D-myo-inositol hexakisphosphate
UDP-glucose + D-fructose 6-phosphate = UDP + sucrose 6(F)-phosphate
2 ATP = 2 diphosphate + cyclic di-3',5'-adenylate
Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose
(1a) ATP + [DNA ligase]-L-lysine = 5'-adenosyl [DNA ligase]-N(epsilon)-phosphono-L-lysine + diphosphate
dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + H(2)O
UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose
Geranylgeranyl diphosphate = (+)-copalyl diphosphate
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate
Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the alpha- and beta-limit dextrins of amylopectin and glycogen.
Prenyl phosphate + FMNH(2) = prenylated FMNH(2) + phosphate
(S)-lactate + NAD(+) = pyruvate + NADH
NAD(P)H + O(2) = NAD(P)(+) + H(2)O(2)
8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16 phosphate
L-proline + NAD(P)(+) = 1-pyrroline-5-carboxylate + NAD(P)H
Cleavage of peptide bonds with very broad specificity.
Release of N-terminal proline from a peptide.
Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
A nucleoside triphosphate + H(2)O = a nucleotide + diphosphate
A long-chain aldehyde + O(2) + 2 NADPH = an alkane + formate + H(2)O + 2 NADP(+)
5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate
ATP = 3',5'-cyclic AMP + diphosphate
Hydrolyzes glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or Gly) for the enzyme from tobacco etch virus. The natural substrate is the viral polyprotein, but other proteins and oligopeptides containing the appropriate consensus sequence are also cleaved.
Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.
Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolyzed is itself substituted at C-3.
Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
H(2) + NAD(+) = H(+) + NADH
Acetyl-CoA + a 2-deoxystreptamine antibiotic = CoA + N(3)-acetyl-2-deoxystreptamine antibiotic
ATP + (L-Asp(4-L-Arg))(n)-L-Asp + L-Arg = ADP + phosphate + (L-Asp(4-L-Arg))(n+1)
A primary alcohol + NAD(+) = an aldehyde + NADH
Glycerone phosphate = methylglyoxal + phosphate
RH + H(2)O(2) = ROH + H(2)O
Prephenate = phenylpyruvate + H(2)O + CO(2)
5,10-methylenetetrahydrofolate + dUMP + NADPH = dTMP + tetrahydrofolate + NADP(+)
L-aspartate = D-aspartate
A 4-O-methyl-D-glucopyranuronate ester + H(2)O = 4-O-methyl-D-glucuronic acid + an alcohol
A (3R)-3-hydroxyacyl-[acyl-carrier-protein] + a UDP-3-O-((3R)-hydroxyacyl)-alpha-D-glucosamine = a UDP-2-N,3-O-bis((3R)-3-hydroxyacyl)-alpha-D-glucosamine + a holo-[acyl-carrier-protein]
S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical
GDP-beta-L-fucose + NADP(+) = GDP-4-dehydro-alpha-D-rhamnose + NADPH
Triphosphate + [microsomal-membrane protein] = diphosphate + [microsomal-membrane protein] phosphate
Hydrolyzes poly(ADP-D-ribose) at glycosidic (1''-2') linkage of ribose-ribose bond to produce free ADP-D-ribose
S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]
Acetyl-CoA + H(2)O + oxaloacetate = citrate + CoA
A beta-D-glucuronoside + H(2)O = D-glucuronate + an alcohol
ATP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide = ADP + phosphate + NADH
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
A nitroalkane + H(2)O + O(2) = an aldehyde or ketone + nitrite + H(2)O(2)
UDP-glucose + 2 NAD(+) + H(2)O = UDP-glucuronate + 2 NADH
S-adenosyl-L-methionine + a 5-hydroxyfurocoumarin = S-adenosyl-L-homocysteine + a 5-methoxyfurocoumarin
ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys)
A phosphate monoester + H(2)O = an alcohol + phosphate
3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholan-24-oylglycine + H(2)O = 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholanate + glycine
Oligoglycosylglucosyl-(1<->1)-ceramide + H(2)O = ceramide + oligoglycosylglucose
2 reduced ferredoxin + NADP(+) + H(+) = 2 oxidized ferredoxin + NADPH
Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides
Cleaves proteins of the adenovirus and its host cell at two consensus sites: -Yaa-Xaa-Gly-Gly-|-Xaa- and -Yaa-Xaa-Gly-Xaa-|-Gly- (in which Yaa is Met, Ile or Leu, and Xaa is any amino acid).
Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.
S-formylglutathione + H(2)O = glutathione + formate
SsDNA + n NTP = ssDNA/pppN(pN)(n-1) hybrid + (n-1) diphosphate
(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate
3'-phosphoadenylyl sulfate + a phenol = adenosine 3',5'-bisphosphate + an aryl sulfate
S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine
A (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a (Z)-3-oxooctadec-11-enoyl-[acyl-carrier protein] + CO(2) + an [acyl-carrier protein]
Cleaves -Ala-|-Ser- and -Ala-|-Ala- bonds in the scaffold protein.
3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO(2)
Hydrolysis of proteins and small molecule substrates at -Asn-|-Xaa- bonds.
Endonucleolytic cleavage of RNA, removing 21 and 42 nucleotides, respectively, from the 5'- and 3'-termini of a 5S-rRNA precursor.
Endonucleolytic cleavage to 5'-phosphomonoester.
ATP + beta-nicotinate-D-ribonucleotide = diphosphate + deamido-NAD(+)
Cyclohexanone + NADPH + O(2) = hexano-6-lactone + NADP(+) + H(2)O
(1a) ATP + [RNA ligase]-L-lysine = [RNA ligase]-N(6)-(5'-adenylyl)-L-lysine + diphosphate
ATP + UMP = ADP + UDP
D-glucose 6-phosphate = D-fructose 6-phosphate
D-ribose 5-phosphate = D-ribulose 5-phosphate
2 nitric oxide + 2 O(2) + NAD(P)H = 2 nitrate + NAD(P)(+) + H(+)
Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate
Beta-nicotinate D-ribonucleotide + diphosphate + CO(2) = pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate
(1a) [E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [RBR-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [RBR-type E3 ubiquitin transferase]-S-ubiquitinyl-L-cysteine
ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
A chalcone = a flavanone
4 ferrocytochrome c + O(2) + 4 H(+) = 4 ferricytochrome c + 2 H(2)O
(1a) L-methionine = methanethiol + 2-aminobut-2-enoate
Glycolate + NADP(+) = glyoxylate + NADPH
D-glycerate + NAD(P)(+) = hydroxypyruvate + NAD(P)H
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
NH(3) + 2 H(2)O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H(+)
dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-beta-L-rhamnose
1-(5-phospho-beta-D-ribosyl)-AMP + H(2)O = 1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide
2 pyruvate = 2-acetolactate + CO(2)
DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine
5,10-methenyltetrahydrofolate + H(2)O = 10-formyltetrahydrofolate
Cytosine + H(2)O = uracil + NH(3)
5,10-methylenetetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH
Quercetin + O(2) = 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO + H(+)
1-(5-phospho-beta-D-ribosyl)-ATP + H(2)O = 1-(5-phospho-beta-D-ribosyl)-AMP + diphosphate
Cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA)
NADH + ROOH + H(+) = NAD(+) + H(2)O + ROH
Sedoheptulose 1,7-bisphosphate + H(2)O = sedoheptulose 7-phosphate + phosphate
Maltose = alpha,alpha-trehalose
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units
(S)-lactaldehyde + NAD(+) + H(2)O = (S)-lactate + NADH
L-histidine-[translation elongation factor 2] + S-adenosyl-L-methionine = 2-((3S)-3-amino-3-carboxypropyl)-L-histidine-[translation elongation factor 2] + S-methyl-5'-thioadenosine
Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val
(1a) 1-C-(indol-3-yl)glycerol 3-phosphate = indole + D-glyceraldehyde 3-phosphate
ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate
Trypanothione + NADP(+) = trypanothione disulfide + NADPH
ATP + RNA(n) = diphosphate + RNA(n+1)
(1a) [acetyl-CoA C-acyltransferase]-S-acyl-L-cyteine + acetyl-CoA = 3-oxoacyl-CoA + [acetyl-CoA C-acyltransferase]-L-cyteine
Methylamine + H(2)O + 2 oxidized [amicyanin] = formaldehyde + NH(3) + 2 reduced [amicyanin]
Isovaleryl-CoA + electron-transfer flavoprotein = 3-methylcrotonyl-CoA + reduced electron-transfer flavoprotein
An aldehyde + NAD(P)(+) + H(2)O = a carboxylate + NAD(P)H
4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate
Eliminative cleavage of alginate to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enuronosyl groups at their non-reducing ends and beta-D-mannuronate at their reducing end.
L-aspartate = beta-alanine + CO(2)
L-glutamate = 4-aminobutanoate + CO(2)
D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH
UDP-alpha-D-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate
2-C-methyl-D-erythritol 4-phosphate + NADP(+) = 1-deoxy-D-xylulose 5-phosphate + NADPH
CTP + sn-glycerol 3-phosphate = diphosphate + CDP-glycerol
Hydrolysis of terminal non-reducing alpha-L-rhamnose residues in alpha-L-rhamnosides
S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate = S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate
NAD(+) + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine
(S)(+)-allantoin = (R)(-)-allantoin
A 5'-phosphopolynucleotide + H(2)O = a polynucleotide + phosphate
Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides
ADP-D-ribose 1''-phosphate + H(2)O = ADP-D-ribose + phosphate
Acetaldehyde + CoA + NAD(+) = acetyl-CoA + NADH
(S)-ureidoglycolate = glyoxylate + urea
A pyrimidine 5'-nucleotide + H(2)O = D-ribose 5-phosphate + a pyrimidine base
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
((1->4)-alpha-D-glucosyl)(n) + phosphate = ((1->4)-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate
(1a) ATP + [DNA ligase]-L-lysine = [DNA ligase]-N(6)-(5'-adenylyl)-L-lysine + diphosphate
ATP + thiamine = AMP + thiamine diphosphate
CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
UDP-alpha-D-glucuronate + NAD(+) = UDP-beta-L-threo-pentapyranos-4-ulose + CO(2) + NADH
Endohydrolysis of (1->4)-beta-D-linkages between D-galactose 4-sulfate and 3,6-anhydro-D-galactose in kappa-carrageenans.
(1a) L-glutamine + H(2)O = L-glutamate + NH(4)(+)
Alpha-D-mannose 1-phosphate = D-mannose 6-phosphate
Acetyl-CoA + H(2)O + glyoxylate = (S)-malate + CoA
4-fumarylacetoacetate + H(2)O = acetoacetate + fumarate
GTP + 3 H(2)O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH
ATP + D-fructose = ADP + D-fructose 6-phosphate
Orotidine 5'-phosphate = UMP + CO(2)
D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate
D-glucarate = 5-dehydro-4-deoxy-D-glucarate + H(2)O
Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate
5-carboxymethyl-2-hydroxymuconate = (3E,5R)-5-carboxy-2-oxohept-3-enedioate
Hydrolysis of --Pro-|- and to a lesser extent --Ala-|- in oligopeptides.
(1a) (2R,3S)-3-isopropylmalate = 2-isopropylmaleate + H(2)O
10-formyltetrahydrofolate + UDP-4-amino-4-deoxy-beta-L-arabinose = 5,6,7,8-tetrahydrofolate + UDP-4-deoxy-4-formamido-beta-L-arabinose
6-carboxy-5,6,7,8-tetrahydropterin = 7-carboxy-7-carbaguanine + NH(3)
S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine(34) in tRNA(Leu) = S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine(34) in tRNA(Leu)
L-lysyl-tRNA(Lys) + phosphatidylglycerol = tRNA(Lys) + 3-O-L-lysyl-1-O-phosphatidylglycerol
ATP + AMP = 2 ADP
ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate
(1a) [enzyme]-L-histidine + 2,3-bisphospho-D-glycerate = [enzyme]-N(tau)-phospho-L-histidine + 2/3-phospho-D-glycerate
N-acylsphingosine + H(2)O = a carboxylate + sphingosine
Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.
D-arabinose 5-phosphate = D-ribulose 5-phosphate
L-ornithine = L-proline + NH(3)
All bonds known to be hydrolyzed by this endopeptidase have arginine in P1 and an acidic residue in P4. P6 is often occupied by an acidic residue or by a hydroxy-amino-acid residue, the phosphorylation of which enhances cleavage.
Anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD(+) = 2-(4-dimethylaminophenyl)diazenylbenzoate + 2 NADH
Coproporphyrinogen-III + O(2) + 2 H(+) = protoporphyrinogen-IX + 2 CO(2) + 2 H(2)O
L-glutaminyl-peptide = 5-oxoprolyl-peptide + NH(3)
[Molybdopterin-synthase sulfur-carrier protein]-Gly-Gly-AMP + [cysteine desulfurase]-S-sulfanyl-L-cysteine + reduced acceptor = AMP + [molybdopterin-synthase sulfur-carrier protein]-Gly-NH-CH(2)-C(O)SH + [cysteine desulfurase] + oxidized acceptor
Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO(2) + [acyl-carrier-protein]
CO + H(2)O + 2 oxidized ferredoxin = CO(2) + 2 reduced ferredoxin + 2 H(+)
Oleoyl-[acyl-carrier-protein] + H(2)O = [acyl-carrier-protein] + oleate
S-methyl-5'-thioadenosine + H(2)O = 5-(methylsulfanyl)-D-ribose + adenine
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine(36) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(36)
A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H(2)O
ATP + [molybdopterin-synthase sulfur-carrier protein]-Gly-Gly = diphosphate + [molybdopterin-synthase sulfur-carrier protein]-Gly-Gly-AMP
Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Acetyl-CoA + n malonyl-CoA + 2n NADPH = a long-chain fatty acid + (n+1) CoA + n CO(2) + 2n NADP(+)
Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein]
Acetyl-CoA + [acyl-carrier-protein] = CoA + acetyl-[acyl-carrier-protein]
3-beta-D-glucosyl-D-glucose + phosphate = D-glucose + alpha-D-glucose 1-phosphate
[Protein]-N(pi)-phospho-L-histidine + D-mannose(Side 1) = [protein]-L-histidine + D-mannose 6-phosphate(Side 2)
2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate
(1a) dTDP-beta-L-evernosamine + NADPH + O(2) = dTDP-N-hydroxy-beta-L-evernosamine + NADP(+) + H(2)O
O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate
Release of an N-terminal dipeptide from a peptide comprising four or more residues, with broad specificity. Also acts on dipeptidyl 2-naphthylamides.
1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate = pyridoxine 5'-phosphate + phosphate + 2 H(2)O
D-galactonate = 2-dehydro-3-deoxy-D-galactonate + H(2)O
D-sorbitol 6-phosphate + NAD(+) = D-fructose 6-phosphate + NADH
(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)(+) + H(2)O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H
Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
ATP + pantetheine 4'-phosphate = diphosphate + 3'-dephospho-CoA
Succinate semialdehyde + NAD(P)(+) + H(2)O = succinate + NAD(P)H
Selective cleavage of Arg-|-Lys bond in complement factor B when in complex with complement subcomponent C3b or with cobra venom factor.
ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)
N-acetyl-D-glucosamine 6-phosphate + H(2)O = D-glucosamine 6-phosphate + acetate
Acetyl-CoA + L-2,4-diaminobutanoate = CoA + N(4)-acetyl-L-2,4-diaminobutanoate
1D-chiro-inositol + NAD(+) = 2D-2,3,5/4,6-pentahydroxycyclohexanone + NADH
Myo-inositol + NAD(+) = 2,4,6/3,5-pentahydroxycyclohexanone + NADH
ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe)
Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.
Cyclizes part of a (1->6)-alpha-D-glucan chain by formation of a (1->6)-alpha-D-glucosidic bond
2 H(2)O + 2 plastoquinone + 4 light = O(2) + 2 plastoquinol
2 5-aminolevulinate = porphobilinogen + 2 H(2)O
ATP + H(2)O + H(+)(Side 1) = ADP + phosphate + H(+)(Side 2)
N(6)-(Delta(2)-isopentenyl)-adenosine 5'-phosphate + H(2)O = N(6)-(dimethylallyl)adenine + D-ribose 5'-phosphate
3 ATP = pppA2'p5'A2'p5'A + 2 diphosphate
Phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
2'-deoxycytidine + H(2)O = 2'-deoxyuridine + NH(3)
ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp)
ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate
S-adenosyl-L-methionine + cytidine(32) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(32) in tRNA
Glycerol + NAD(+) = glycerone + NADH
6-phospho-D-glucono-1,5-lactone + H(2)O = 6-phospho-D-gluconate
ATP + (d)CMP = ADP + (d)CDP
2 S-adenosyl-L-methionine + adenine(2503) in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine(2503) in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin
ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate
(1a) glutaryl-CoA + electron-transfer flavoprotein = (E)-glutaconyl-CoA + reduced electron-transfer flavoprotein
A 3-oxosteroid + acceptor = a 3-oxo-Delta(1)-steroid + reduced acceptor
ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide
D-fructose 1,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate
ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole
(+)-muconolactone = cis,cis-muconate
Alpha-maltose 1-phosphate + ((1->4)-alpha-D-glucosyl)(n) = phosphate + ((1->4)-alpha-D-glucosyl)(n+2)
(S)-mandelate = (R)-mandelate
Maleate = fumarate
An aryl sulfate + H(2)O = a phenol + sulfate
Succinate semialdehyde + NADP(+) + H(2)O = succinate + NADPH
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]
((1->2)-beta-D-glucosyl)(n) + H(2)O = sophorose + ((1->2)-beta-D-glucosyl)(n-2)
Isochorismate + H(2)O = (2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate
UDP-alpha-D-galactopyranose = UDP-alpha-D-galactofuranose
An archaeal dolichyl phosphooligosaccharide + [protein]-L-asparagine = an archaeal dolichyl phosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine
An aryl sulfate + a phenol = a phenol + an aryl sulfate
N(6)-(L-1,3-dicarboxypropyl)-L-lysine + NAD(+) + H(2)O = L-lysine + 2-oxoglutarate + NADH
4-(2-carboxyphenyl)-4-oxobutanoyl-CoA = 1,4-dihydroxy-2-naphthoyl-CoA + H(2)O
GTP + a 5'-diphospho-[mRNA] = diphosphate + a 5'-(5'-triphosphoguanosine)-[mRNA]
O-phospho-L-homoserine + H(2)O = L-threonine + phosphate
L-arabinose = L-ribulose
dCTP + H(2)O = dCMP + diphosphate
UDP-glucose + (1,4-beta-D-glucosyl)(n) = UDP + (1,4-beta-D-glucosyl)(n+1)
5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H(2)O = 5-formyl-5,6,7,8-tetrahydromethanopterin
Formyl-CoA + oxalate = formate + oxalyl-CoA
(R)-mandelonitrile = cyanide + benzaldehyde
ATP + sulfate = diphosphate + adenylyl sulfate
ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec)
ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr)
NAD(+) + glycine + sulfide = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H(2)O
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO(3)(-) = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole
Angiotensin II + H(2)O = angiotensin-(1-7) + L-phenylalanine
Xylitol + NADP(+) = L-xylulose + NADPH
D-xylopyranose = D-xylulose
Urate + O(2) + H(2)O = 5-hydroxyisourate + H(2)O(2)
L-methionine + thioredoxin disulfide + H(2)O = L-methionine (S)-S-oxide + thioredoxin
Preferential cleavage at the carboxyl of hydrophobic amino acids, but fails to cleave 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17 and 24-Phe-|-Phe-25 of insulin B chain. Activates trypsinogen, and degrades keratin.
Phosphoenolpyruvate + D-arabinose 5-phosphate + H(2)O = 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
S-adenosyl-L-methionine + 3-((1-carboxyvinyl)oxy)benzoate + H(2)O = 6-amino-6-deoxyfutalosine + L-methionine + HCO(3)(-)
Uroporphyrinogen III = coproporphyrinogen + 4 CO(2)
ATP + L-pyrrolysine + tRNA(Pyl) = AMP + diphosphate + L-pyrrolysyl-tRNA(Pyl)
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
L-homoserine + NAD(P)(+) = L-aspartate 4-semialdehyde + NAD(P)H
Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1)
L-tyrosine + 2-oxoglutarate = 4-hydroxyphenylpyruvate + L-glutamate
Chorismate = isochorismate
CTP + (R)-4'-phosphopantothenate + L-cysteine = CMP + diphosphate + N-((R)-4'-phosphopantothenoyl)-L-cysteine
The peptide bond hydrolyzed can be designated -C-|-A-A-X in which C is an S-isoprenylated cysteine residue, A is usually aliphatic and X is the C-terminal residue of the substrate protein, and may be any of several amino acids.
NAD(P)H + a quinone = NAD(P)(+) + a hydroquinone
N-((R)-4'-phosphopantothenoyl)-L-cysteine = pantotheine 4'-phosphate + CO(2)
(2S)-2-hydroxy-3,4-dioxopentyl phosphate = 3-hydroxy-2,4-dioxopentyl phosphate
ATP + 3-methylbut-3-en-1-yl phosphate = ADP + 3-methylbut-3-en-1-yl diphosphate
An L-amino acid = a D-amino acid
Phenylacetaldehyde + NAD(+) + H(2)O = phenylacetate + NADH
Eliminative cleavage of the terminal beta-D-mannosyl-(1->4)-beta-D-glucuronosyl linkage of the side-chain of the polysaccharide xanthan, leaving a 4-deoxy-alpha-L-threo-hex-4-enuronosyl group at the terminus of the side-chain
ATP + thiamine phosphate = ADP + thiamine diphosphate
(1a) acetyl-CoA + [acetyl-CoA C-acetyltransferase]-L-cysteine = [acetyl-CoA C-acetyltransferase]-S-acetyl-L-cysteine + CoA
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
(R)-mevalonate + CoA + 2 NAD(+) = 3-hydroxy-3-methylglutaryl-CoA + 2 NADH
4 sulfur + 4 H(2)O + O(2) = 2 H(2)S + 2 HSO(3)(-) + 2 H(+)
L-allo-threonine = glycine + acetaldehyde
S-adenosyl-L-methionine + precorrin-5 + H(2)O = S-adenosyl-L-homocysteine + precorrin-6A + acetate
D-mannitol + NAD(+) = D-fructose + NADH
ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione
S-adenosyl-L-methionine + [peptide chain release factor 1 or 2]-L-glutamine = S-adenosyl-L-homocysteine + [peptide chain release factor 1 or 2]-N(5)-methyl-L-glutamine
Glutarate + 2-oxoglutarate + O(2) = (S)-2-hydroxyglutarate + succinate + CO(2)
Hydrolysis of the 1,3-alpha-L-galactosidic linkages of neoagaro-oligosaccharides that are smaller than a hexamer, yielding 3,6-anhydro-L-galactose and D-galactose
2 glutathione + NADP(+) = glutathione disulfide + NADPH
L-lysine + O(2) + H(2)O = 6-amino-2-oxohexanoate + NH(3) + H(2)O(2)
ATP + L-fuculose = ADP + L-fuculose 1-phosphate
ATP + H(2)O + vitamin B12-[cobalamin-binding protein](Side 1) = ADP + phosphate + vitamin B12(Side 2) + [cobalamin-binding protein](Side 1)
L-threonine = glycine + acetaldehyde
D-firefly luciferin + O(2) + ATP = firefly oxyluciferin + CO(2) + AMP + diphosphate + light
L-glutamate + H(2)O + NAD(+) = 2-oxoglutarate + NH(3) + NADH
L-ornithine + a 2-oxo acid = L-glutamate 5-semialdehyde + an L-amino acid
P(1),P(4)-bis(5'-guanosyl) tetraphosphate + H(2)O = GTP + GMP
GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose
UDP-N-acetyl-alpha-D-glucosamine + H(2)O = N-acetyl-D-mannosamine + UDP
Acetyl-CoA + phosphate = CoA + acetyl phosphate
Phosphonoacetaldehyde + H(2)O = acetaldehyde + phosphate
Isocitrate = succinate + glyoxylate
Nicotinamide + H(2)O = nicotinate + NH(3)
(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate = 8 diphosphate + di-trans,octa-cis-undecaprenyl diphosphate
Acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine
ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate
Benzoate + NADH + O(2) = (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD(+)
Release of a C-terminal amino acid with broad specificity, except for -Pro.
A primary alcohol + 2 cytochrome c(L) = an aldehyde + 2 reduced cytochrome c(L) + 2 H(+)
A primary alcohol + 2 cytochrome c = an aldehyde + 2 reduced cytochrome c + 2 H(+)
(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate
5,6-dihydrouracil + H(2)O = 3-ureidopropanoate
(1a) (S)-6-hydroxynicotine + O(2) = 5-(N-methyl-4,5-dihydro-1H-pyrrol-2-yl)pyridin-2-ol + H(2)O(2)
GDP-mannose + 3-phospho-D-glycerate = GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate
n ATP + n H(2)O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers
Endohydrolysis of (1->6)-alpha-D-glucosidic linkages in dextran
[Protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H(2)O = [protein]-L-serine + N-acetyl-D-glucosamine
Feruloyl-polysaccharide + H(2)O = ferulate + polysaccharide
8-oxo-dGTP + H(2)O = 8-oxo-dGMP + diphosphate
2 GTP = 2 diphosphate + cyclic di-3',5'-guanylate
Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|- and the P1' position is occupied by Gly-|-
Endohydrolysis of beta-(1->4)-linkages between D-glucosamine residues in a partly acetylated chitosan
GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP
Hydrolysis of (1->4)-alpha-D-glucosidic linkages in amylaceous polysaccharides, to remove successive maltohexaose residues from the non-reducing chain ends
Arsenate + glutathione + glutaredoxin = arsenite + a glutaredoxin-glutathione disulfide + H(2)O
Preferential cleavage of bonds with hydrophobic residues in P1'.
Alditol + NAD(P)(+) = aldose + NAD(P)H
UDP-N,N'-diacetylbacillosamine + tritrans,heptacis-undecaprenyl phosphate = UMP + N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol
L-glutamate = D-glutamate
L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate
L-ornithine = D-ornithine
4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H(2)O = 4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose
Hydroxymethylbilane = uroporphyrinogen III + H(2)O
(R)-10-hydroxystearate = oleate + H(2)O
4a-hydroxytetrahydrobiopterin = 6,7-dihydrobiopterin + H(2)O
ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine
Nitrous oxide + 2 ferricytochrome c + H(2)O = 2 nitric oxide + 2 ferrocytochrome c + 2 H(+)
A short-chain acyl-CoA + electron-transfer flavoprotein = a short-chain trans-2,3-dehydroacyl-CoA + reduced electron-transfer flavoprotein
L-fuculose 1-phosphate = glycerone phosphate + (S)-lactaldehyde
ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)
4-trimethylammoniobutanoate + 2-oxoglutarate + O(2) = 3-hydroxy-4-trimethylammoniobutanoate + succinate + CO(2) 
5-methyltetrahydrofolate + NAD(P)(+) = 5,10-methylenetetrahydrofolate + NAD(P)H
ATP + 2,3-dihydroxybenzoate = diphosphate + (2,3-dihydroxybenzoyl)adenylate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero 31-mer (preferred)
Entry contents:
29 distinct polypeptide molecules
2 distinct RNA molecules
Macromolecules (31 distinct):
50S ribosomal protein L2 Chain: A
Molecule details ›
Chain: A
Length: 240 amino acids
Theoretical weight: 25.35 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P20276 (Residues: 1-240; Coverage: 100%)
Gene names: rpl2, rrnAC1608
Sequence domains:
Structure domains:
50S ribosomal protein L3 Chain: B
Molecule details ›
Chain: B
Length: 338 amino acids
Theoretical weight: 37.4 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P20279 (Residues: 1-338; Coverage: 100%)
Gene names: rpl3, rrnAC1611
Sequence domains: Ribosomal protein L3
Structure domains:
50S ribosomal protein L4 Chain: C
Molecule details ›
Chain: C
Length: 246 amino acids
Theoretical weight: 26.44 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P12735 (Residues: 1-246; Coverage: 100%)
Gene names: rpl4, rrnAC1610
Sequence domains: Ribosomal protein L4/L1 family
Structure domains: Ribosomal Protein L4; Chain: A;
50S ribosomal protein L5 Chain: D
Molecule details ›
Chain: D
Length: 177 amino acids
Theoretical weight: 19.55 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P14124 (Residues: 1-177; Coverage: 100%)
Gene names: rpl5, rrnAC1598
Sequence domains:
Structure domains: 50s Ribosomal Protein L5; Chain: A,
50S ribosomal protein L6 Chain: E
Molecule details ›
Chain: E
Length: 178 amino acids
Theoretical weight: 19.96 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P14135 (Residues: 1-178; Coverage: 100%)
Gene names: rpl6, rrnAC1596
Sequence domains: Ribosomal protein L6
Structure domains: Ribosomal protein L6, alpha-beta domain
50S ribosomal protein L7Ae Chain: F
Molecule details ›
Chain: F
Length: 120 amino acids
Theoretical weight: 12.79 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P12743 (Residues: 1-120; Coverage: 100%)
Gene names: rpl7ae, rrnAC0103
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Structure domains: 60s Ribosomal Protein L30; Chain: A;
50S ribosomal protein L10 Chain: G
Molecule details ›
Chain: G
Length: 348 amino acids
Theoretical weight: 37.21 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P15825 (Residues: 1-348; Coverage: 100%)
Gene names: rpl10, rpl10e, rplP0, rrnAC1417
Sequence domains:
50S ribosomal protein L10e Chain: H
Molecule details ›
Chain: H
Length: 177 amino acids
Theoretical weight: 19.94 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P60617 (Residues: 1-177; Coverage: 100%)
Gene names: rpl10e, rrnAC2357
Sequence domains: Ribosomal protein L16p/L10e
Structure domains: Ribosomal protein L10e/L16
50S ribosomal protein L11 Chain: I
Molecule details ›
Chain: I
Length: 162 amino acids
Theoretical weight: 17.1 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P14122 (Residues: 1-162; Coverage: 100%)
Gene names: rpl11, rrnAC1414
Sequence domains:
Structure domains: Ribosomal protein L11, C-terminal domain
50S ribosomal protein L13 Chain: J
Molecule details ›
Chain: J
Length: 145 amino acids
Theoretical weight: 16.25 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P29198 (Residues: 1-145; Coverage: 100%)
Gene names: rpl13, rrnAC0065
Sequence domains: Ribosomal protein L13
Structure domains: Ribosomal protein L13
50S ribosomal protein L14 Chain: K
Molecule details ›
Chain: K
Length: 132 amino acids
Theoretical weight: 14.19 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P22450 (Residues: 1-132; Coverage: 100%)
Gene names: rpl14, rrnAC1602
Sequence domains: Ribosomal protein L14p/L23e
Structure domains: Ribosomal protein L14
50S ribosomal protein L15 Chain: L
Molecule details ›
Chain: L
Length: 165 amino acids
Theoretical weight: 18.01 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P12737 (Residues: 1-165; Coverage: 100%)
Gene names: rpl15, rrnAC1590
Sequence domains: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
Structure domains:
50S ribosomal protein L15e Chain: M
Molecule details ›
Chain: M
Length: 195 amino acids
Theoretical weight: 22.28 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P60618 (Residues: 1-195; Coverage: 100%)
Gene names: rpl15e, rrnAC2065
Sequence domains: Ribosomal L15
Structure domains: Ribosomal protein l15e
50S ribosomal protein L18 Chain: N
Molecule details ›
Chain: N
Length: 187 amino acids
Theoretical weight: 20.64 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P14123 (Residues: 1-187; Coverage: 100%)
Gene names: rpl18, rrnAC1593
Sequence domains: Ribosomal large subunit proteins 60S L5, and 50S L18
Structure domains: Nucleotidyltransferase; domain 5
50S ribosomal protein L18e Chain: O
Molecule details ›
Chain: O
Length: 116 amino acids
Theoretical weight: 12.44 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P12733 (Residues: 1-116; Coverage: 100%)
Gene names: rpl18e, rrnAC0064
Sequence domains: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
Structure domains: Ribosomal Protein L15; Chain: K; domain 2
50S ribosomal protein L19e Chain: P
Molecule details ›
Chain: P
Length: 149 amino acids
Theoretical weight: 16.8 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P14119 (Residues: 1-149; Coverage: 100%)
Gene names: rpl19e, rrnAC1594
Sequence domains: Ribosomal protein L19e
Structure domains:
50S ribosomal protein L21e Chain: Q
Molecule details ›
Chain: Q
Length: 96 amino acids
Theoretical weight: 10.57 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P12734 (Residues: 1-96; Coverage: 100%)
Gene names: rpl21e, rrnAC0260
Sequence domains: Ribosomal protein L21e
Structure domains: Ribosomal protein L21
50S ribosomal protein L22 Chain: R
Molecule details ›
Chain: R
Length: 152 amino acids
Theoretical weight: 16.65 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P10970 (Residues: 1-155; Coverage: 98%)
Gene names: rpl22, rrnAC1606
Sequence domains: Ribosomal protein L22p/L17e
Structure domains: Ribosomal protein L22/L17
50S ribosomal protein L23 Chain: S
Molecule details ›
Chain: S
Length: 85 amino acids
Theoretical weight: 9.61 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P12732 (Residues: 1-85; Coverage: 100%)
Gene names: rpl23, rrnAC1609
Sequence domains: Ribosomal protein L23
Structure domains: Alpha-Beta Plaits
50S ribosomal protein L24 Chain: T
Molecule details ›
Chain: T
Length: 120 amino acids
Theoretical weight: 13.67 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P10972 (Residues: 1-120; Coverage: 100%)
Gene names: rpl24, rrnAC1601
Sequence domains:
Structure domains: SH3 type barrels.
50S ribosomal protein L24e Chain: U
Molecule details ›
Chain: U
Length: 66 amino acids
Theoretical weight: 7.23 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P14116 (Residues: 2-67; Coverage: 99%)
Gene names: rpl24e, rrnAC0104
Sequence domains: Ribosomal protein L24e
Structure domains: Ribosomal protein L24e
50S ribosomal protein L29 Chain: V
Molecule details ›
Chain: V
Length: 71 amino acids
Theoretical weight: 7.89 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P10971 (Residues: 1-71; Coverage: 100%)
Gene names: rpl29, rrnAC1604
Sequence domains: Ribosomal L29 protein
Structure domains: Helix Hairpins
50S ribosomal protein L30 Chain: W
Molecule details ›
Chain: W
Length: 154 amino acids
Theoretical weight: 17.06 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P14121 (Residues: 1-154; Coverage: 100%)
Gene names: rpl30, rrnAC1591
Sequence domains: Ribosomal protein L30p/L7e
Structure domains:
50S ribosomal protein L31e Chain: X
Molecule details ›
Chain: X
Length: 92 amino acids
Theoretical weight: 10.38 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P18138 (Residues: 1-92; Coverage: 100%)
Gene names: rpl31e, rrnAC3113
Sequence domains: Ribosomal protein L31e
Structure domains: Ribosomal Protein L31e; Chain: W;
50S ribosomal protein L32e Chain: Y
Molecule details ›
Chain: Y
Length: 241 amino acids
Theoretical weight: 26.46 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P12736 (Residues: 1-241; Coverage: 100%)
Gene names: rpl32e, rrnAC1595
Sequence domains:
50S ribosomal protein L37Ae Chain: Z
Molecule details ›
Chain: Z
Length: 83 amino acids
Theoretical weight: 9.41 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P60619 (Residues: 10-92; Coverage: 90%)
Gene names: rpl37ae, rrnAC1669
Structure domains: N-terminal domain of TfIIb
50S ribosomal protein L37e Chain: 1
Molecule details ›
Chain: 1
Length: 57 amino acids
Theoretical weight: 6.33 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P32410 (Residues: 1-57; Coverage: 100%)
Gene names: rpl37e, rrnAC0797.1
Sequence domains: Ribosomal protein L37e
Structure domains: N-terminal domain of TfIIb
50S ribosomal protein L39e Chain: 2
Molecule details ›
Chain: 2
Length: 50 amino acids
Theoretical weight: 6.13 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P22452 (Residues: 1-50; Coverage: 100%)
Gene names: rpl39e, rrnAC3112
Sequence domains: Ribosomal L39 protein
Structure domains: Ribosomal protein L39e
50S ribosomal protein L44e Chain: 3
Molecule details ›
Chain: 3
Length: 92 amino acids
Theoretical weight: 10.82 KDa
Source organism: Haloarcula marismortui
UniProt:
  • Canonical: P32411 (Residues: 1-92; Coverage: 100%)
Gene names: rpl44e, rrnAC3514
Sequence domains: Ribosomal protein L44
Structure domains: Nuclear Transport Factor 2; Chain: A,
23S ribosomal RNA Chain: 0
5S ribosomal RNA Chain: 9
Molecule details ›
Chain: 9
Length: 122 nucleotides
Theoretical weight: 39.3 KDa
Sequence domains: 5S ribosomal RNA

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2221
Unit cell:
a: 213.076Å b: 300.75Å c: 575.478Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.184 0.242