1ygu

X-ray diffraction
2.9Å resolution

Crystal structure of the tandem phosphatase domains of RPTP CD45 with a pTyr peptide

Released:

Function and Biology Details

Reaction catalysed:
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Receptor-type tyrosine-protein phosphatase C Chains: A, B
Molecule details ›
Chains: A, B
Length: 610 amino acids
Theoretical weight: 71.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P08575 (Residues: 624-1233; Coverage: 48%)
Gene names: CD45, PTPRC
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily
Middle T antigen Chains: C, D
Molecule details ›
Chains: C, D
Length: 4 amino acids
Theoretical weight: 546 Da
Source organism: Murine polyomavirus strain A2
Expression system: Not provided
UniProt:
  • Canonical: P03077 (Residues: 248-251; Coverage: 1%)

Ligands and Environments

No bound ligands
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21
Unit cell:
a: 84.9Å b: 57.92Å c: 159.29Å
α: 90° β: 98.66° γ: 90°
R-values:
R R work R free
0.265 0.262 0.305
Expression systems:
  • Escherichia coli BL21
  • Not provided