1ygr

X-ray diffraction
2.9Å resolution

Crystal structure of the tandem phosphatase domain of RPTP CD45

Released:

Function and Biology Details

Reaction catalysed:
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Receptor-type tyrosine-protein phosphatase C Chains: A, B
Molecule details ›
Chains: A, B
Length: 610 amino acids
Theoretical weight: 71.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08575 (Residues: 624-1233; Coverage: 48%)
Gene names: CD45, PTPRC
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily
T-cell surface glycoprotein CD3 zeta chain Chains: C, D
Molecule details ›
Chains: C, D
Length: 6 amino acids
Theoretical weight: 891 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P20963 (Residues: 80-85; Coverage: 4%)
Gene names: CD247, CD3Z, T3Z, TCRZ

Ligands and Environments

No bound ligands
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21
Unit cell:
a: 86.01Å b: 59.7Å c: 160.53Å
α: 90° β: 99.53° γ: 90°
R-values:
R R work R free
0.261 0.255 0.312
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided